囊性纤维化患者痰液中的鞭毛蛋白浓度。
Flagellin concentrations in expectorations from cystic fibrosis patients.
作者信息
Balloy Viviane, Thévenot Guiti, Bienvenu Thierry, Morand Philippe, Corvol Harriet, Clement Annick, Ramphal Reuben, Hubert Dominique, Chignard Michel
机构信息
Unité de Défense innée et Inflammation, Institut Pasteur, Paris, France.
出版信息
BMC Pulm Med. 2014 Jun 9;14:100. doi: 10.1186/1471-2466-14-100.
BACKGROUND
The aim was to measure flagellin concentrations in the expectorations of CF patients and to examine whether there are correlations with the level of respiratory insufficiency and inflammation.
METHODS
Sputum samples from 31 adult patients chronically colonized with P. aeruginosa were collected and analysed for their content of flagellin and IL-8. Clinical data were extracted from patient files.
RESULTS
Regardless of whether patients are colonized with mucoid strains or not, they carry clones of P. aeruginosa that express flagellin. While flagellin was present in airways of all of our CF patients, it is difficult to ascertain its contribution to inflammation (IL-8) and lung function deterioration.
CONCLUSIONS
This is the first demonstration that flagellin is present in the sputum of patients. Thus, attempts to down regulate inflammation by the use of TLR5 (flagellin receptor) antagonists remain a possibility. However, this result needs to be extended to a larger number of patients to validate it for future research on this subject.
背景
目的是测量囊性纤维化（CF）患者痰液中的鞭毛蛋白浓度，并检查其与呼吸功能不全和炎症水平是否存在相关性。
方法
收集31例长期被铜绿假单胞菌定植的成年患者的痰液样本，分析其中鞭毛蛋白和白细胞介素-8（IL-8）的含量。临床数据从患者病历中提取。
结果
无论患者是否被黏液样菌株定植，他们都携带表达鞭毛蛋白的铜绿假单胞菌克隆。虽然我们所有CF患者的气道中都存在鞭毛蛋白，但很难确定其对炎症（IL-8）和肺功能恶化的影响。
结论
这是首次证明鞭毛蛋白存在于患者痰液中。因此，使用Toll样受体5（TLR5，鞭毛蛋白受体）拮抗剂下调炎症反应仍有可能。然而，这一结果需要扩大到更多患者进行验证，以便为该主题的未来研究提供依据。
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