Liu Mengna, Xie Jingyan, Zhao Shuli
Department of Gynecology, Nanjing Hospital, Nanjing Medical University, Nanjing 210006, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Jun;30(6):627-9, 634.
To investigate the relationships and implications between the various immune cell subsets in the peripheral blood of patients with ovarian cancer and the clinicopathological parameters.
Flow cytometry (FCM) was used to test the regulatory T cells (Tregs), helper T cells (Th1 cells), dendritic cells (DCs), natural killer (NK) cells and other immune cell subsets in the pre-operative peripheral blood and ascites of 24 patients with malignant ovarian tumors. Then, the results were compared with those in the peripheral blood of 20 healthy women, and their relationships with the clinicopathological parameters were analyzed statistically.
Compared with the control group, there were an elevated level of Tregs, significantly reduced levels of Th1 cells and activated receptor NKG2D on CD8⁺ T cells (P<0.05) in the peripheral blood of the 24 patients with ovarian cancer, which were correlated with pathological staging (P<0.05). The total DCs were not significantly different between healthy controls and ovarian cancer patients, but mature DCs (mDCs) were reduced and plasmacytoid DCs (pDCs) increased in the peripheral blood of the patients with ovarian cancer. The degree of the decreased mDCs was related to clinical stage, and the degree of the increased pDCs was correlated with pathological grade (P<0.05). Besides, compared with peripheral blood, the levels of CD16 on NK cells and NKG2D receptor on CD8⁺ T cells dropped in ascites.
The anti-tumor immune function declined in patients with malignant ovarian tumors, and the immune functions of the tumor-killing effect T cells and NK cells in the peripheral blood and ascites of the patients were both significantly inhibited, meanwhile, the antigen presentation of DCs was descended.
探讨卵巢癌患者外周血中各种免疫细胞亚群与临床病理参数之间的关系及意义。
采用流式细胞术（FCM）检测24例恶性卵巢肿瘤患者术前外周血及腹水中的调节性T细胞（Tregs）、辅助性T细胞（Th1细胞）、树突状细胞（DCs）、自然杀伤（NK）细胞等免疫细胞亚群。然后，将结果与20名健康女性外周血中的结果进行比较，并对它们与临床病理参数的关系进行统计学分析。
与对照组相比，24例卵巢癌患者外周血中Tregs水平升高，Th1细胞水平及CD8⁺T细胞上活化受体NKG2D水平显著降低（P<0.05），且与病理分期相关（P<0.05）。健康对照组与卵巢癌患者之间总DCs无显著差异，但卵巢癌患者外周血中成熟DCs（mDCs）减少，浆细胞样DCs（pDCs）增加。mDCs减少的程度与临床分期有关，pDCs增加的程度与病理分级相关（P<0.05）。此外，与外周血相比，腹水中NK细胞上CD16水平及CD8⁺T细胞上NKG2D受体水平下降。
恶性卵巢肿瘤患者的抗肿瘤免疫功能下降，患者外周血及腹水中具有肿瘤杀伤作用的T细胞和NK细胞的免疫功能均受到显著抑制，同时DCs的抗原呈递功能下降。
