Yang G, Forrest R, Zhou H, Hodge S, Hickford J
Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Science, Lanzhou, China.
J Anim Breed Genet. 2014 Dec;131(6):437-44. doi: 10.1111/jbg.12097. Epub 2014 Jun 9.
The uncoupling protein 1 (UCP1) plays an important role in the regulation of lipolysis and thermogenesis in adipose tissues. Genetic variation within three regions (the promoter, intron 2 and exon 5) of the ovine UCP1 gene (UCP1) was investigated using polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) analyses. These revealed three promoter variants (designated A, B and C) and two intron 2 variants (a and b). The association of this genetic variation with variation in lamb carcass traits and postweaning growth was investigated in New Zealand (NZ) Romney and Suffolk sheep. The presence of B in a lamb's genotype was associated with decreased subcutaneous carcass fat depth (V-GR) (p = 0.004) and proportion of total lean meat yield of loin meat (p = 0.005), and an increased proportion of total lean meat yield of hind-leg meat (p = 0.018). In contrast, having two copies of C was associated with increased V-GR (p < 0.001) and proportion of total lean meat yield of shoulder meat (p = 0.009), and a decreased hind-leg yield (p = 0.032). No associations were found with postweaning growth. These results suggest that ovine UCP1 is a potential gene marker for carcass traits.
解偶联蛋白1（UCP1）在脂肪组织的脂解作用和产热调节中发挥着重要作用。利用聚合酶链反应-单链构象多态性（PCR-SSCP）分析，研究了绵羊UCP1基因（UCP1）三个区域（启动子、内含子2和外显子5）内的遗传变异。这些分析揭示了三种启动子变体（分别命名为A、B和C）和两种内含子2变体（a和b）。在新西兰罗姆尼羊和萨福克羊中，研究了这种遗传变异与羔羊胴体性状变异和断奶后生长之间的关联。羔羊基因型中存在B与皮下胴体脂肪深度降低（V-GR）（p = 0.004）以及腰肉总瘦肉产量比例降低（p = 0.005）相关，而后腿肉总瘦肉产量比例增加（p = 0.018）。相反，具有两个C拷贝与V-GR增加（p < 0.001）、肩部肉总瘦肉产量比例增加（p = 0.009）以及后腿产量降低（p = 0.032）相关。未发现与断奶后生长有关联。这些结果表明，绵羊UCP1是一种潜在的胴体性状基因标记。
