利用反式环辛烯与四嗪的生物正交反应对RNA进行位点特异性荧光标记。
Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine.
作者信息
Asare-Okai P N, Agustin E, Fabris D, Royzen M
机构信息
Department of Chemistry, University at Albany, SUNY, 1400 Washington Avenue, Albany, NY 12222, USA.
出版信息
Chem Commun (Camb). 2014 Jul 25;50(58):7844-7. doi: 10.1039/c4cc02435d.
PMID:24909672
Abstract
This communication describes a general approach for site-specific fluorescence labelling of RNA using a cytidine triphosphate (CTP) analogue derivatized with a trans-cyclooctene group. The analogue was efficiently incorporated into a model RNA strand using in vitro transcription. Bio-orthogonal reaction with fluorescein-labelled tetrazine was utilized to fluorescently tag the synthetic RNA strand.
摘要
本通讯描述了一种使用经反式环辛烯基团衍生化的三磷酸胞苷（CTP）类似物对RNA进行位点特异性荧光标记的通用方法。该类似物通过体外转录被有效地掺入到模型RNA链中。利用与荧光素标记的四嗪进行的生物正交反应对合成的RNA链进行荧光标记。
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