一氧化氮介导的人羊膜间充质干细胞对小胶质细胞活力和迁移的免疫抑制作用。
Nitric oxide-mediated immunosuppressive effect of human amniotic membrane-derived mesenchymal stem cells on the viability and migration of microglia.
作者信息
Yan Ke, Zhang Run, Chen Lei, Chen Fanfan, Liu Yi, Peng Lingmei, Sun Haitao, Huang Weiyi, Sun Chengmei, Lv Bingke, Li Feng, Cai Yingqian, Tang Yanping, Zou Yuxi, Du Mouxuan, Qin Lingsha, Zhang Hengzhu, Jiang Xiaodan
机构信息
The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China; Department of Neurosurgery, Clinical Medical College of Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
出版信息
Brain Res. 2014 Nov 24;1590:1-9. doi: 10.1016/j.brainres.2014.05.041. Epub 2014 Jun 6.
Human amniotic membrane-derived mesenchymal stem cells (AMSCs) are considered a novel and promising source of stem cells for cell replacement-based therapy. Current research is mostly limited to investigating the cellular differentiation potential of AMSCs, while few have focused on their immunosuppressive properties. This study is aimed at exploring and evaluating the immunosuppressive effect of human AMSCs on the viability and migratory properties of microglia. We found, from results of cell viability assays, that AMSCs can reduce the activity of inflammatory cells by secreting nitric oxide (NO). Also, based on results from wound healing and transwell migration assays, we show that AMSCs can inhibit the migration of human microglia as well as the mouse microglial cell line BV2, suggesting that they have the ability to inhibit the recruitment of certain immune cells to injury sites. Furthermore, we found that NO contributes significantly to this inhibitory effect. Our study provides evidence that human AMSCs can have detrimental effects on the viability and migration of microglia, through secretion of NO. This mechanism may contribute to anti-inflammatory processes in the central nervous system.
人羊膜间充质干细胞（AMSCs）被认为是一种用于基于细胞替代疗法的新型且有前景的干细胞来源。目前的研究大多局限于探究AMSCs的细胞分化潜能，而很少关注其免疫抑制特性。本研究旨在探索和评估人AMSCs对小胶质细胞活力和迁移特性的免疫抑制作用。我们从细胞活力测定结果中发现，AMSCs可通过分泌一氧化氮（NO）来降低炎症细胞的活性。此外，基于伤口愈合和Transwell迁移测定的结果，我们表明AMSCs可抑制人小胶质细胞以及小鼠小胶质细胞系BV2的迁移，这表明它们有能力抑制某些免疫细胞向损伤部位的募集。此外，我们发现NO对这种抑制作用有显著贡献。我们的数据表明，人AMSCs可通过分泌NO对小胶质细胞的活力和迁移产生有害影响。这一机制可能有助于中枢神经系统的抗炎过程。
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