Guo Wei, Feng Jinglan, Song Hong, Sun Jianhui
School of Environment, Key Laboratory for Yellow River and Huaihe River Water Environmental and Pollution Control Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, Henan Normal University, Xinxiang, 453007, People's Republic of China.
Environ Sci Pollut Res Int. 2014 Oct;21(19):11531-40. doi: 10.1007/s11356-014-3071-9. Epub 2014 Jun 10.
The objectives of this study were to investigate the simultaneous bioelectricity generation and decolorization of methyl orange (MO) in the anode chamber of microbial fuel cells (MFCs) in a wide concentration range (from 50 to 800 mg L(-1)) and to reveal the microbial communities on the anode after the MFC was operated continuously for more than 6 months using MO-glucose mixtures as fuel. Interestingly, the added MO played an active role in the production of electricity. The maximum voltage outputs were 565, 658, 640, 629, 617, and 605 mV for the 1 g L(-1) glucose with 0, 50, 100, 200, 300, and 500 mg L(-1) of MO, respectively. The results of three groups of comparison experiments showed that accelerated decolorization of methyl orange (MO) was achieved in the MFC as compared to MFC in open circuit mode and MFC without extra carbon sources. The decolorization efficiency decreased with an increase of MO concentration in the studied concentration range for the dye load increased. A 454 high-throughput pyrosequencing revealed the microbial communities. Geobacter genus known to generate electricity was detected. Bacteroidia class, Desulfovibrio, and Trichococcus genus, which were most likely responsible for degrading methyl orange, were also detected.
本研究的目的是在较宽的浓度范围（50至800 mg L(-1)）内，研究微生物燃料电池（MFCs）阳极室中甲基橙（MO）的同步生物发电和脱色情况，并揭示以MO-葡萄糖混合物为燃料连续运行6个月以上后阳极上的微生物群落。有趣的是，添加的MO在发电过程中发挥了积极作用。对于分别含有0、50、100、200、300和500 mg L(-1) MO的1 g L(-1)葡萄糖，最大电压输出分别为565、658、640、629、617和605 mV。三组对比实验结果表明，与开路模式下的MFC和无额外碳源的MFC相比，MFC中甲基橙（MO）的脱色加速。在所研究的浓度范围内，随着MO浓度的增加，脱色效率下降，因为染料负荷增加。454高通量焦磷酸测序揭示了微生物群落。检测到已知能发电的地杆菌属。还检测到最有可能负责降解甲基橙的拟杆菌纲、脱硫弧菌属和四联球菌属。
