Miran Waheed, Nawaz Mohsin, Kadam Avinash, Shin Seolhye, Heo Jun, Jang Jiseon, Lee Dae Sung
Department of Environmental Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, Republic of Korea.
Environ Sci Pollut Res Int. 2015 Sep;22(17):13477-85. doi: 10.1007/s11356-015-4582-8. Epub 2015 May 5.
The expansion in knowledge of the microbial community structure can play a vital role in the electrochemical features and operation of microbial fuel cells (MFCs). In this study, bacterial community composition in a dual chamber MFC fed with brewery waste was investigated for simultaneous electricity generation and azo dye degradation. A stable voltage was generated with a maximum power density of 305 and 269 mW m(-2) for brewery waste alone (2000 mg L(-1)) and after the azo dye (200 mg L(-1)) addition, respectively. Azo dye degradation was confirmed by Fourier transform infrared spectroscopy (FT-IR) as peak corresponding to -N=N- (azo) bond disappeared in the dye metabolites. Microbial communities attached to the anode were analyzed by high-throughput 454 pyrosequencing of the 16S rRNA gene. Microbial community composition analysis revealed that Proteobacteria (67.3 %), Betaproteobacteria (30.8 %), and Desulfovibrio (18.3 %) were the most dominant communities at phylum, class, and genus level, respectively. Among the classified genera, Desulfovibrio most likely plays a major role in electron transfer to the anode since its outer membrane contains c-type cytochromes. At the genus level, 62.3 % of all sequences belonged to the unclassified category indicating a high level of diversity of microbial groups in MFCs fed with brewery waste and azo dye.
• Azo dye degradation and stable bioelectricity generation was achieved in the MFC. • Anodic biofilm was analyzed by high-throughput pyrosequencing of the 16S rRNA gene. • Desulfovibrio (18.3 %) was the dominant genus in the classified genera. • Of the genus, 62.3 % were unclassified, thereby indicating highly diverse microbes. Graphical Abstract A schematic diagram of a dual chamber microbial fuel cell for azo dye degradation and current generation (with microbial communities at anode electrode).
微生物群落结构知识的扩展对微生物燃料电池（MFC）的电化学特性和运行起着至关重要的作用。在本研究中，对以啤酒厂废水为进料的双室MFC中的细菌群落组成进行了研究，以实现同时发电和偶氮染料降解。仅以啤酒厂废水（2000 mg L(-1)）为进料时以及添加偶氮染料（200 mg L(-1)）后，分别产生了稳定的电压，最大功率密度分别为305和269 mW m(-2)。通过傅里叶变换红外光谱（FT-IR）确认了偶氮染料的降解，因为染料代谢产物中对应于-N=N-（偶氮）键的峰消失了。通过对16S rRNA基因进行高通量454焦磷酸测序分析附着在阳极上的微生物群落。微生物群落组成分析表明，在门、纲和属水平上，变形菌门（67.3%）、β-变形菌纲（30.8%）和脱硫弧菌属（18.3%）是最主要的群落。在已分类的属中，脱硫弧菌属最有可能在向阳极的电子转移中起主要作用，因为其外膜含有c型细胞色素。在属水平上，所有序列的62.3%属于未分类类别，这表明在以啤酒厂废水和偶氮染料为进料的MFC中微生物群体具有高度的多样性。
• 在MFC中实现了偶氮染料降解和稳定的生物发电。• 通过对16S rRNA基因进行高通量焦磷酸测序分析阳极生物膜。• 脱硫弧菌属（18.3%）是已分类属中的优势属。• 在该属中，62.3%未分类，从而表明微生物高度多样。图形摘要 用于偶氮染料降解和电流产生的双室微生物燃料电池示意图（阳极电极处有微生物群落）
