Ravi Maddaly, Paramesh V, Kaviya S R, Anuradha E, Solomon F D Paul
Department of Human Genetics, Faculty of Biomedical Sciences, Technology and Research, Sri Ramachandra University, Porur, Chennai, India.
J Cell Physiol. 2015 Jan;230(1):16-26. doi: 10.1002/jcp.24683.
Cell cultures are important material of study for the variety of advantages that they offer. Both established continuous cell lines and primary cell cultures continue to be invaluable for basic research and for direct applications. Technological advancements are necessary to address emerging complex challenges and the way cells are cultured in vitro is an area of intense activity. One important advancement in cell culture techniques has been the introduction of three dimensional culture systems. This area is one of the fastest growing experimental approaches in life sciences. Augmented with advancements in cell imaging and analytical systems, as well as the applications of new scaffolds and matrices, cells have been increasingly grown as three dimensional models. Such cultures have proven to be closer to in vivo natural systems, thus proving to be useful material for many applications. Here, we review the three dimensional way of culturing cells, their advantages, the scaffolds and matrices currently available, and the applications of such cultures in major areas of life sciences.
细胞培养因其所具有的多种优势而成为重要的研究材料。已建立的连续细胞系和原代细胞培养对于基础研究和直接应用而言仍然具有不可估量的价值。为应对新出现的复杂挑战，技术进步必不可少，而细胞的体外培养方式正是一个活跃的研究领域。细胞培养技术的一项重要进展是三维培养系统的引入。该领域是生命科学中发展最快的实验方法之一。随着细胞成像和分析系统的进步，以及新型支架和基质的应用，细胞越来越多地被培养成三维模型。事实证明，这种培养方式更接近体内自然系统，因此成为许多应用的有用材料。在此，我们综述细胞的三维培养方式、其优势、目前可用的支架和基质，以及这种培养方式在生命科学主要领域的应用。
