恶性疟原虫cDNA文库的构建与筛选

Construction and screening of Plasmodium falciparum cDNA library.

作者信息

Cheng Q, Shen Y F, Qiang B Q, Yu S H, Wu N H, Chen J F, Fan R G, Cheng X K, Li W L, Mao Y H

机构信息

Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.

出版信息

Chin J Biotechnol. 1989;5(2):65-71.

PMID:2491316

Abstract

The HAINAN isolate of Plasmodium falciparum FCC1/HN was cultured in vitro in large quantities. The total parasite mRNA was purified and reverse transcribed into cDNA. The cDNA fragments were inserted into lambda gt11 to construct a P. falciparum FCC1/HN erythrocytic stage cDNA library. Inhibitory monoclonal antibodies (McAbs) M26-32, F6-C2, and F6-D3 were used to screen the cDNA library expressed in E. coli. A total of 27 positive clones were found to react with M26-32 alone and 34 clones with both M26-32 and F6-C2. These expressed proteins may be candidates for use in malaria vaccine.

摘要

恶性疟原虫海南分离株FCC1/HN在体外大量培养。纯化总寄生虫mRNA并反转录成cDNA。将cDNA片段插入λgt11构建恶性疟原虫FCC1/HN红细胞期cDNA文库。用抑制性单克隆抗体（McAbs）M26-32、F6-C2和F6-D3筛选在大肠杆菌中表达的cDNA文库。共发现27个阳性克隆仅与M26-32反应，34个克隆与M26-32和F6-C2均反应。这些表达的蛋白可能是疟疾疫苗的候选物。