Wu L J, Liu E X, Zhu Z Y, Miao W M
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1993;11(1):25-8.
Plasmodium falciparum FCC1/HN-infected human erythrocytes were embedded with LR White resin at low temperature. The 185 kDa and 82/41 kDa proteins in erythrocytic stages of P. falciparum were then immunolabeled by using the protective monoclonal antibodies (McAb)F6-D3 and F6-C2 with protein A-colloidal gold probe. The electron-microscopical observation showed that the 185kDa protein recognized by McAb F6-D3 was located on the surface of free and intracellular merozoites as well as the cytoplasm, plasma membrane, and parasitophorous vacuole membrane of immature schizonts. The 82/41 kDa proteins identified by McAb F6-C2 was located within the rhoptries of immature schizonts and mature merozoites. These results demonstrated ultrastructurally that the 185 kDa and 82/41 kDa protective antigens were merozoite surface antigen and merozoite rhoptry antigens of P. falciparum FCC1/HN, respectively.
恶性疟原虫FCC1/HN感染的人红细胞在低温下用LR White树脂包埋。然后，使用保护性单克隆抗体（McAb）F6-D3和F6-C2与蛋白A-胶体金探针，对恶性疟原虫红细胞期的185 kDa和82/41 kDa蛋白进行免疫标记。电子显微镜观察显示，McAb F6-D3识别的185 kDa蛋白位于游离和细胞内裂殖子的表面，以及未成熟裂殖体的细胞质、质膜和寄生泡膜上。McAb F6-C2鉴定的82/41 kDa蛋白位于未成熟裂殖体和成熟裂殖子的棒状体中。这些结果在超微结构上表明，185 kDa和82/41 kDa保护性抗原分别是恶性疟原虫FCC1/HN的裂殖子表面抗原和裂殖子棒状体抗原。
