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吸水链霉菌应城变种与庆丰链霉菌的种间原生质体融合及其重组体的生物学特性

Interspecific protoplast fusion of Streptomyces hygroscopicus var. yingchengenisis with Streptomyces qingfengmyceticus and biological characterization of their recombinants.

作者信息

Zhou X F, Zhou Q

机构信息

Agricultural Antibiotic Laboratory, Huazhong Agricultural University, Wuhan.

出版信息

Chin J Biotechnol. 1989;5(3):161-6.

PMID:2491325
Abstract

The frequency of recombination between Streptomyces hygroscopicus var. yingchengensis and Streptomyces qingfengmyceticus as a result of interspecific protoplast fusion was 4.5 x 10(-4). Characterization of biological properties revealed differences in spore mass color, drug resistance, antimicrobial activity, and antibiotic production capacity between the recombinants and their parents. Two recombinant strains, FL-42 and FL-48, exhibited production of the antibiotics originally elaborated by the two parents individually. They also produced two compounds with antimicrobial activity that their parents could not synthesize.

摘要

吸水链霉菌应城变种与庆丰链霉菌间原生质体融合产生的重组频率为4.5×10⁻⁴。生物学特性表征显示,重组体与其亲本在孢子堆颜色、耐药性、抗菌活性及抗生素生产能力方面存在差异。两个重组菌株FL - 42和FL - 48分别产生了最初由两个亲本各自合成的抗生素。它们还产生了两种具有抗菌活性的化合物,而其亲本无法合成这些化合物。

相似文献

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Interspecific protoplast fusion of Streptomyces hygroscopicus var. yingchengenisis with Streptomyces qingfengmyceticus and biological characterization of their recombinants.吸水链霉菌应城变种与庆丰链霉菌的种间原生质体融合及其重组体的生物学特性
Chin J Biotechnol. 1989;5(3):161-6.
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引用本文的文献

1
Development of a gene cloning system for Streptomyces hygroscopicus subsp. yingchengensis, a producer of three useful antifungal compounds, by elimination of three barriers to DNA transfer.通过消除DNA转移的三个障碍,开发一种用于吸水链霉菌应城亚种(三种有用抗真菌化合物的产生菌)的基因克隆系统。
J Bacteriol. 1994 Apr;176(7):2090-5. doi: 10.1128/jb.176.7.2090-2095.1994.