Yang H Y, Zhou B G, Sun L Q, Yang J P, Mai Y C
Bethune University of Medical Sciences, Changchun.
Chin J Biotechnol. 1989;5(3):167-71.
Five monoclonal antibodies (McAbs) against ovine prolactin (oPRL) were obtained by the lymphocyte hybridoma technique. One low-affinity McAb with (2D2) was purified by hydroxylapatite chromatography. The 2D2 was immobilized on Sepharose 4B with epichlorohydrin to produce an immunosorbent column, which was used to purify oPRL from a crude preparation by a single-step procedure.
