Purification of ovine prolactin by affinity chromatography with monoclonal antibodies.
作者信息
Yang H Y, Zhou B G, Sun L Q, Yang J P, Mai Y C
机构信息
Bethune University of Medical Sciences, Changchun.
出版信息
Chin J Biotechnol. 1989;5(3):167-71.
PMID:2491326
Abstract
Five monoclonal antibodies (McAbs) against ovine prolactin (oPRL) were obtained by the lymphocyte hybridoma technique. One low-affinity McAb with (2D2) was purified by hydroxylapatite chromatography. The 2D2 was immobilized on Sepharose 4B with epichlorohydrin to produce an immunosorbent column, which was used to purify oPRL from a crude preparation by a single-step procedure.
摘要
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