Yu Y M, Yu M Z, Shi Y X
Institute of Developmental Biology, Academia Sinica, Beijing.
Chin J Biotechnol. 1989;5(2):81-8.
Immunized spleen lymphocytes were fused with Balb/c mouse myeloma cells to obtain hybridoma cell line 4B-2, which produces monoclonal antibodies (McAb) against bovine growth hormone (bGH). Balb/c mice were injected with 4B-2 cells to produce ascites antibodies. The specific McAb in the ascites (about 10 mg/ml, subclass IgG1) was purified by immunoaffinity chromatography (IAC) on bGH-Sepharose 4B. This McAb cross-reacted with porcine growth hormone (pGH) but not with human (hGH), ovine (oGH), or fish (fGH) growth hormone. A 17 mg portion of purified McAb, coupled to the IAC affinity column was used to purify 1 mg of bGH. The bGH preparation retained its activity in rabbit liver receptor assay and in the tibia test following IAC.
将免疫的脾淋巴细胞与Balb/c小鼠骨髓瘤细胞融合，获得杂交瘤细胞系4B - 2，该细胞系产生抗牛生长激素（bGH）的单克隆抗体（McAb）。给Balb/c小鼠注射4B - 2细胞以产生腹水抗体。腹水中的特异性McAb（约10 mg/ml，亚类IgG1）通过在bGH - Sepharose 4B上的免疫亲和色谱（IAC）进行纯化。该McAb与猪生长激素（pGH）发生交叉反应，但与人类（hGH）、绵羊（oGH）或鱼类（fGH）生长激素不发生交叉反应。将17 mg纯化的McAb偶联到IAC亲和柱上，用于纯化1 mg的bGH。在IAC后，bGH制剂在兔肝受体测定和胫骨试验中保留了其活性。
