Shi Y A, Shen L P, Tang X X, Li Z P, Yuan W K, Chen M H
East China University of Chemical Technology, Shanghai.
Chin J Biotechnol. 1989;5(4):203-11.
Both cell growth and expression of hepatitis B surface antigen (HBsAg) interact with each other in fermentation to produce exogenous protein HBsAg by genetically engineered yeast, S. cerevisiae, which contains the HBsAg gene. Since both processes required proper pH values in order to improve the overall yield, a suitable pH value sequence was determined. It is important that dissolved oxygen concentration in fermentation should be controlled to maintain the stability of recombinant plasmids and to obtain the high HBsAg expression. When the concentration of dissolved oxygen was controlled at 70% saturation, the stability of recombinant plasmid was maintained at 73%, and the relative concentration of HBsAg expression was found to be 98.6.
在通过含有乙肝表面抗原（HBsAg）基因的基因工程酵母酿酒酵母发酵生产外源蛋白HBsAg的过程中，细胞生长和HBsAg的表达相互作用。由于这两个过程都需要合适的pH值来提高总产量，因此确定了合适的pH值序列。重要的是，应控制发酵中的溶解氧浓度，以维持重组质粒的稳定性并获得高HBsAg表达。当溶解氧浓度控制在70%饱和度时，重组质粒的稳定性维持在73%，并且发现HBsAg表达的相对浓度为98.6。
