对Sr9h的特性描述，Sr9h是一个对Ug99有效的小麦抗秆锈病等位基因。

Characterization of Sr9h, a wheat stem rust resistance allele effective to Ug99.

作者信息

Rouse Matthew N, Nirmala Jayaveeramuthu, Jin Yue, Chao Shiaoman, Fetch Thomas G, Pretorius Zacharias A, Hiebert Colin W

机构信息

Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), 1551 Lindig Street, St. Paul, MN, USA,

出版信息

Theor Appl Genet. 2014 Aug;127(8):1681-8. doi: 10.1007/s00122-014-2330-y. Epub 2014 Jun 10.

DOI:10.1007/s00122-014-2330-y

Abstract

Wheat stem rust resistance gene SrWeb is an allele at the Sr9 locus that confers resistance to Ug99. Race TTKSK (Ug99) of Puccinia graminis f. sp. tritici, the causal fungus of stem rust, threatens global wheat production because of its broad virulence to current wheat cultivars. A recently identified Ug99 resistance gene from cultivar Webster, temporarily designated as SrWeb, mapped near the stem rust resistance gene locus Sr9. We determined that SrWeb is also present in Ug99 resistant cultivar Gabo 56 by comparative mapping and an allelism test. Analysis of resistance in a population segregating for both Sr9e and SrWeb demonstrated that SrWeb is an allele at the Sr9 locus, which subsequently was designated as Sr9h. Webster and Gabo 56 were susceptible to the Ug99-related race TTKSF+ from South Africa. Race TTKSF+ possesses unique virulence to uncharacterized Ug99 resistance in cultivar Matlabas. This result validated that resistance to Ug99 in Webster and Gabo 56 is conferred by the same gene: Sr9h. The emergence of pathogen virulence to several resistance genes that are effective to the original Ug99 race TTKSK, including Sr9h, suggests that resistance genes should be used in combinations in order to increase resistance durability.

摘要

小麦抗秆锈病基因SrWeb是位于Sr9位点的一个等位基因，可赋予对Ug99的抗性。秆锈病的致病真菌——小麦秆锈菌的TTKSK小种（Ug99），因其对当前小麦品种具有广泛的毒性，威胁着全球小麦生产。最近从品种Webster中鉴定出一个Ug99抗性基因，暂命名为SrWeb，它定位在秆锈病抗性基因位点Sr9附近。通过比较作图和等位性测试，我们确定SrWeb也存在于Ug99抗性品种Gabo 56中。对同时分离Sr9e和SrWeb的群体进行抗性分析表明，SrWeb是Sr9位点的一个等位基因，随后被命名为Sr9h。Webster和Gabo 56对来自南非的与Ug99相关的TTKSF+小种敏感。TTKSF+小种对品种Matlabas中未鉴定的Ug99抗性具有独特的毒性。这一结果证实，Webster和Gabo 56对Ug99的抗性是由同一个基因Sr9h赋予的。包括Sr9h在内的几个对原始Ug99小种TTKSK有效的抗性基因出现了病原体毒性，这表明抗性基因应组合使用以提高抗性持久性。