Simultaneous determination of 17 disperse dyes in textile by ultra-high performance supercritical fluid chromatography combined with tandem mass spectrometry.

A simple, highly sensitive and fast procedure for the control of 17 allergenic and prohibited disperse dyes in textile products was optimized. The method was based on ultrasound assisted extraction of textile samples with 10 mL of methanol under controlled conditions (30 min, 70°C). The extracts were analyzed by the ultra-high performance supercritical fluid chromatography (UHPSFC) system coupled with triple quadrupole tandem mass spectrometry (MS/MS). Four stationary phases (BEH, BEH 2-ethyl-pyridine, HSS C18 SB and CSH fluorophenyl) were screened as well as analytical conditions (modifier percentage, backpressure and column temperature) were investigated to improve the separation. All 17 disperse dyes were simultaneously separated and determined by UHPSFC-MS/MS in 5 min. The dyes were monitored via the ESI(+) ionization method and quantified by 3-channel multiple reaction monitoring (MRM). The calibrations were performed and good linear relationship (R≥0.99) was observed within the concentration range of 2-50 μg mL(-1). Satisfactory recoveries (70.55-103.03%) of all the disperse dyes spiked with standards at different levels were demonstrated. This is the first report on the simultaneous analysis of disperse dyes using UHPSFC-MS/MS.