Ding Youchao, Cao Xizhong, Wu Lina, Zhang Qian
Inspection Center of Industrial Products, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China.
Se Pu. 2008 Sep;26(5):603-7.
A qualitative method of the identification of nine carcinogenic dyes prohibited in textiles was developed using high performance liquid chromatography-tandem mass spectrometry interfaced with electro-spray ionization (HPLC-ESI-MS/MS) in the selective reaction monitoring (SRM) mode. The dyes were extracted from textiles composed of natural or chemical fibers by methanol under ultrasounication, and then eluted with gradient by acetonitrile and 5 mmol/L ammonium acetate from an RP-C18 column with two segments in effluents. The first effluents accommodated Acid Red 26, Direct Blue 6, Direct Black 38 and Direct Red 28 with negative ionization mode, while the second accommodated Basic Red 9, Basic Violet 14, Disperse Blue 1, Disperse Orange 11 and Disperse Yellow 3 with positive ionization mode. Thus the investigated compounds could be identified simultaneously with single-run analysis no matter which type of the fibre the sample was and no matter which category of the dye the analyte was. The established method was successfully applied to identify the carcinogenic dyes in textile samples through comparing the chromatographic retention time and the relative abundance of characteristic product ions with the standards.
采用高效液相色谱-串联质谱联用技术（HPLC-ESI-MS/MS），在选择性反应监测（SRM）模式下，建立了一种定性鉴定纺织品中九种禁用致癌染料的方法。通过甲醇在超声条件下从天然或化学纤维组成的纺织品中提取染料，然后用乙腈和5 mmol/L醋酸铵从RP-C18柱上进行梯度洗脱，流出物分两段。第一段流出物在负离子模式下容纳酸性红26、直接蓝6、直接黑38和直接红28，而第二段在正离子模式下容纳碱性红9、碱性紫14、分散蓝1、分散橙11和分散黄3。因此，无论样品是哪种纤维类型，也无论分析物是哪种染料类别，都可以通过单次运行分析同时鉴定所研究的化合物。通过将色谱保留时间和特征产物离子的相对丰度与标准品进行比较，所建立的方法成功应用于鉴定纺织品样品中的致癌染料。
