Skråmo Silje, Hersleth Hans Petter, Hammerstad Marta, Andersson K Kristoffer, Røhr Åsmund K
Department of Biosciences, Section for Biochemistry and Molecular Biology, University of Oslo, PO Box 1066, Blindern, NO-0316 Oslo, Norway.
Acta Crystallogr F Struct Biol Commun. 2014 Jun;70(Pt 6):777-80. doi: 10.1107/S2053230X14008334. Epub 2014 May 10.
Ferredoxin/flavodoxin-NADP(H) oxidoreductases (FNRs) are key enzymes involved in catalysing electron transfer between ferredoxins/flavodoxins and NAD(P)H/NAD(P)+. In Bacillus cereus there are three genes that may encode FNRs, and the Bc0385 FNR has been cloned, overexpressed, purified and successfully crystallized in its NADPH/NADP+-free form. Diffraction data have been collected to 2.5 Å resolution from crystals belonging to the orthorhombic space group P2₁2₁2, with unit-cell parameters a=57.2, b=164.3, c=95.0 Å, containing two FNR molecules in the asymmetric unit. The structure of the Bc0385 FNR has been solved by molecular replacement, and is a member of the homodimeric thioredoxin reductase-like class of FNRs.
铁氧化还原蛋白/黄素氧化还原蛋白-NADP(H)氧化还原酶（FNRs）是催化铁氧化还原蛋白/黄素氧化还原蛋白与NAD(P)H/NAD(P)+之间电子转移的关键酶。在蜡样芽孢杆菌中有三个基因可能编码FNRs，并且Bc0385 FNR已被克隆、过表达、纯化，并以其无NADPH/NADP+的形式成功结晶。已从属于正交空间群P2₁2₁2的晶体收集到分辨率为2.5 Å的衍射数据，其晶胞参数a = 57.2、b = 164.3、c = 95.0 Å，不对称单位中包含两个FNR分子。Bc0385 FNR的结构已通过分子置换解析，它是FNRs中同二聚体硫氧还蛋白还原酶样类别的成员。
