枯草芽孢杆菌yumC编码的铁氧化还原蛋白-NADP⁺氧化还原酶的结晶及初步X射线研究
Crystallization and preliminary X-ray studies of ferredoxin-NADP+ oxidoreductase encoded by Bacillus subtilis yumC.
作者信息
Komori Hirofumi, Seo Daisuke, Sakurai Takeshi, Higuchi Yoshiki
机构信息
Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt 3):301-3. doi: 10.1107/S1744309110000151. Epub 2010 Feb 24.
Abstract
Ferredoxin-NADP(+) oxidoreductase encoded by Bacillus subtilis yumC has been purified and successfully crystallized in complex with NADP(+) in two forms. Diffraction data from crystals of these two forms were collected at resolutions of 1.8 and 1.9 A. The former belonged to space group P2(1)2(1)2, with unit-cell parameters a = 63.90, b = 135.72, c = 39.19 A, and the latter to space group C2, with unit-cell parameters a = 207.47, b = 64.85, c = 61.12 A, beta = 105.82 degrees. The initial structure was determined by the molecular-replacement method using a thioredoxin reductase-like protein as a search model.
摘要
枯草芽孢杆菌yumC编码的铁氧化还原蛋白-NADP(+)氧化还原酶已被纯化，并成功以两种形式与NADP(+)形成复合物结晶。这两种形式晶体的衍射数据在1.8和1.9埃分辨率下收集。前者属于空间群P2(1)2(1)2，晶胞参数a = 63.90，b = 135.72，c = 39.19埃，后者属于空间群C2，晶胞参数a = 207.47，b = 64.85，c = 61.12埃，β = 105.82°。初始结构通过分子置换法，以硫氧还蛋白还原酶样蛋白作为搜索模型确定。
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