Cranston Laura J, Roszak Aleksander W, Cogdell Richard J
Institute of Molecular Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, 120 University Place, Glasgow G12 8TA, Scotland.
Acta Crystallogr F Struct Biol Commun. 2014 Jun;70(Pt 6):808-13. doi: 10.1107/S2053230X14009303. Epub 2014 May 24.
LH2 from the purple photosynthetic bacterium Marichromatium (formerly known as Chromatium) purpuratum is an integral membrane pigment-protein complex that is involved in harvesting light energy and transferring it to the LH1-RC `core' complex. The purified LH2 complex was crystallized using the sitting-drop vapour-diffusion method at 294 K. The crystals diffracted to a resolution of 6 Å using synchrotron radiation and belonged to the tetragonal space group I4, with unit-cell parameters a=b=109.36, c=80.45 Å. The data appeared to be twinned, producing apparent diffraction symmetry I422. The tetragonal symmetry of the unit cell and diffraction for the crystals of the LH2 complex from this species reveal that this complex is an octamer.
来自紫色光合细菌紫硫红假单胞菌（以前称为着色菌属）的LH2是一种整合膜色素蛋白复合物，参与收集光能并将其转移到LH1-RC“核心”复合物。纯化的LH2复合物在294 K下使用坐滴气相扩散法结晶。使用同步辐射，晶体衍射至6 Å的分辨率，属于四方晶系空间群I4，晶胞参数a=b=109.36，c=80.45 Å。数据似乎存在孪晶，产生表观衍射对称性I422。该物种LH2复合物晶体的晶胞四方对称性和衍射表明该复合物是八聚体。
