Saijo Shinya, Sato Takao, Kumasaka Takashi, Tanaka Nobuo, Harata Kazuaki, Odahara Takayuki
Department of Life Science, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):83-6. doi: 10.1107/S1744309104028945. Epub 2004 Dec 2.
The reaction center-light-harvesting 1 (RC-LH1) core complex is the photosynthetic apparatus in the membrane of the purple photosynthetic bacterium Rhodopseudomonas viridis. The RC is surrounded by an LH1 complex that is constituted of oligomers of three types of apoproteins (alpha, beta and gamma chains) with associated bacteriochlorophyll bs and carotenoid. It has been crystallized by the sitting-drop vapour-diffusion method. A promising crystal diffracted to beyond 8.0 A resolution. It belonged to space group P1, with unit-cell parameters a = 141.4, b = 136.9, c = 185.3 A, alpha = 104.6, beta = 94.0, gamma = 110.7 degrees. A Patterson function calculated using data between 15.0 and 8.0 A resolution suggested that the LH1 complex is distributed with quasi-16-fold rotational symmetry around the RC.
反应中心-光捕获1（RC-LH1）核心复合物是绿色红假单胞菌紫色光合细菌膜中的光合装置。反应中心被一个LH1复合物包围，该复合物由三种脱辅基蛋白（α、β和γ链）的寡聚体与相关的细菌叶绿素b和类胡萝卜素组成。它已通过坐滴气相扩散法结晶。一个有前景的晶体衍射分辨率超过8.0 Å。它属于空间群P1，晶胞参数a = 141.4，b = 136.9，c = 185.3 Å，α = 104.6，β = 94.0，γ = 110.7°。使用15.0至8.0 Å分辨率之间的数据计算的帕特森函数表明，LH1复合物围绕反应中心以准16倍旋转对称分布。
