Weber Ursula, Rodriguez Estefania, Martignetti John, Mlodzik Marek
Department of Developmental and Regenerative Biology and Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
PLoS One. 2014 Jun 10;9(6):e96933. doi: 10.1371/journal.pone.0096933. eCollection 2014.
Krüppel like factors (KLFs) are conserved transcription factors that have been implicated in many developmental processes including differentiation, organ patterning, or regulation of stem cell pluripotency. We report the generation and analysis of loss-of-function mutants of Drosophila Klf6/7, the luna gene. We demonstrate that luna mutants are associated with very early embryonic defects prior to cellularization at the syncytial stage and cause DNA separation defects during the rapid mitotic cycles resulting in un-coupled DNA and centrosome cycles. These defects manifest themselves, both in animals that are maternally homozygous and heterozygous mutant. Surprisingly, luna is only required during the syncytial stages and not later in development, suggesting that the DNA segregation defect is linked to centrosomes, since centrosomes are dispensable for later cell divisions.
克鲁ppel样因子（KLFs）是保守的转录因子，参与了许多发育过程，包括分化、器官模式形成或干细胞多能性的调控。我们报告了果蝇Klf6/7（即luna基因）功能丧失突变体的产生和分析。我们证明，luna突变体与合胞体阶段细胞化之前的非常早期的胚胎缺陷有关，并在快速有丝分裂周期中导致DNA分离缺陷，从而导致DNA和中心体周期解偶联。这些缺陷在母本纯合和杂合突变的动物中均有表现。令人惊讶的是，luna仅在合胞体阶段是必需的，而在发育后期则不是必需的，这表明DNA分离缺陷与中心体有关，因为中心体对于后期的细胞分裂是可有可无的。
