Department of Biotechnology, Lund University, Lund, Sweden.
Department of Biotechnology, Lund University, Lund, Sweden; Indienz AB, Annebergs Gård PL 5520, Se-26873 Billeberga, Sweden.
J Chromatogr A. 2014 Aug 29;1357:24-35. doi: 10.1016/j.chroma.2014.05.055. Epub 2014 May 27.
Cryogels are polymeric materials formed from monomeric or polymeric precursors in moderately frozen state by polymerization or crosslinking. The process applied for cryogel formation is called cryogelation. These macroporous gel matrices can be produced with different shapes and the gels are of interest in the bioseparation area since they can meet needs that conventional chromatographic media are less suitable to fulfill. High porosity, high mechanical and chemical stability make them appropriate carriers for immobilization of biomolecules and cells thereby making them attractive gel matrices for separation and purification of various molecules. This review highlights the preparation and properties of cryogels, and applications of these materials especially in bioseparation science.
冷冻凝胶是由单体或聚合物前体在适度冷冻状态下通过聚合或交联形成的聚合材料。用于形成冷冻凝胶的过程称为冷冻凝胶化。这些大孔凝胶基质可以用不同的形状制成，并且由于它们可以满足传统色谱介质不太适合满足的需求，因此在生物分离领域很有吸引力。高孔隙率、高机械和化学稳定性使它们成为生物分子和细胞固定化的合适载体，从而使它们成为分离和纯化各种分子的有吸引力的凝胶基质。本文综述了冷冻凝胶的制备和性质，以及这些材料特别是在生物分离科学中的应用。
