聚合物冷冻凝胶作为具有生物技术应用前景的材料。

Polymeric cryogels as promising materials of biotechnological interest.

作者信息

Lozinsky Vladimir I, Galaev Igor Yu, Plieva Fatima M, Savina Irina N, Jungvid Hans, Mattiasson Bo

机构信息

A.N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov St. 28, 119991 Moscow, Russia.

出版信息

Trends Biotechnol. 2003 Oct;21(10):445-51. doi: 10.1016/j.tibtech.2003.08.002.

DOI:10.1016/j.tibtech.2003.08.002

PMID:14512231

Abstract

Cryogels are gel matrices that are formed in moderately frozen solutions of monomeric or polymeric precursors. Cryogels typically have interconnected macropores (or supermacropores), allowing unhindered diffusion of solutes of practically any size, as well as mass transport of nano- and even microparticles. The unique structure of cryogels, in combination with their osmotic, chemical and mechanical stability, makes them attractive matrices for chromatography of biological nanoparticles (plasmids, viruses, cell organelles) and even whole cells. Polymeric cryogels are efficient carriers for the immobilization of biomolecules and cells.

摘要

冷冻凝胶是在单体或聚合物前体的适度冷冻溶液中形成的凝胶基质。冷冻凝胶通常具有相互连接的大孔（或超大孔），允许几乎任何大小的溶质不受阻碍地扩散，以及纳米甚至微粒的质量传输。冷冻凝胶的独特结构，加上它们的渗透、化学和机械稳定性，使其成为用于生物纳米颗粒（质粒、病毒、细胞器）甚至全细胞色谱分析的有吸引力的基质。聚合物冷冻凝胶是固定生物分子和细胞的有效载体。