Kim Jun-Ran, Perumalsamy Haribalan, Kwon Min Jung, Chae Se Um, Ahn Young-Joon
Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Pest Manag Sci. 2015 May;71(5):737-43. doi: 10.1002/ps.3843. Epub 2014 Jul 4.
Dermatophagoides farinae and Tyrophagus putrescentiae are recognised as an important source of allergens. An assessment was made of the toxicity of hiba, Thujopsis dolabrata var. hondai, oil and 13 organic compounds and the control efficacy of four experimental spray formulations containing the oil (5-30 g L(-1) spray) against both mite species.
In a contact + fumigant mortality bioassay, (-)-thujopsene was the most toxic constituent against D. farinae and T. putrescentiae (24 h LC50 9.82 and 10.92 µg cm(-2)), and the toxicity of the compound was nearly identical to that of benzyl benzoate (9.33 and 10.14 µg cm(-2)). The toxicity was more pronounced in carvacrol, (+)-terpinen-4-ol, β-thujaplicin, (-)-terpinen-4-ol, cedrol and α-terpineol (LC50 12.05-15.20 and 12.74-16.48 µg cm(-2)) than in N,N-diethyl-3-methylbenzamide (LC50 35.53 and 38.42 µg cm(-2)) against both mite species. The hiba oil 30 g L(-1) spray and commercial permethrin (cis:trans 25:75) 2.5 g L(-1) spray treatment resulted in 100 and 11% mortality against the two mite species respectively. In vapour-phase mortality tests, the two compounds were consistently more toxic in closed versus open containers, indicating that toxicity was achieved mainly through the action of vapour.
Reasonable mite control in indoor environments can be achieved by a spray formulation containing the 30 g L(-1) hiba oil as a potential contact-action fumigant.
粉尘螨和腐食酪螨被认为是重要的过敏原来源。对日本扁柏（Thujopsis dolabrata var. hondai）油及13种有机化合物的毒性进行了评估，并对含该油（5 - 30 g L⁻¹喷雾）的四种实验性喷雾制剂对这两种螨类的防治效果进行了评估。
在接触 + 熏蒸致死生物测定中，（-）-土荆芥烯对粉尘螨和腐食酪螨毒性最强（24小时LC50分别为9.82和10.92 μg cm⁻²），该化合物的毒性与苯甲酸苄酯（9.33和10.14 μg cm⁻²）几乎相同。香芹酚、（+）-萜品-4-醇、β-崖柏素、（-）-萜品-4-醇、雪松醇和α-萜品醇对两种螨类的毒性（LC50为12.05 - 15.20和12.74 - 16.48 μg cm⁻²）比对二乙甲苯酰胺（LC50为35.53和38.42 μg cm⁻²）更明显。30 g L⁻¹的日本扁柏油喷雾处理和2.5 g L⁻¹的市售氯菊酯（顺式：反式25:75）喷雾处理对这两种螨类的致死率分别为100%和11%。在气相致死试验中，这两种化合物在密闭容器中始终比在开放容器中毒性更强，表明毒性主要通过蒸汽作用实现。
含有30 g L⁻¹日本扁柏油的喷雾制剂作为一种潜在的接触作用熏蒸剂，可在室内环境中实现合理的螨虫防治。
