Steele J K, Levy J G, Waterfield J D
Department of Pathology, Harvard Medical School, Boston, MA 02115.
Autoimmunity. 1989;3(1):17-28. doi: 10.3109/08916938909043610.
We have previously described a monoclonal antibody, B16G, which has been found to be specific for T-cell derived suppressor factors (TsF). B16G has been shown to react with T-suppressor cells, TsF in the spleen of normal or tumor-bearing mice, the TsF produced by tumour-specific, or hapten-specific T-cell hybridomas, and with polyclonal whole human TsF isolated from tonsillar tissue. This pan-reactivity inherent to the B16G antibody suggests that it recognizes some common, shared epitope of the TsF molecule. In this study, we have used B16G as a probe to isolate a TsF-producing T-cell hybridoma, S-50, from CBA mice (H-2k) that is specific for the Lupus-associated antigen, RNP-Sm. The TsF bound specifically to RNP-Sm and inhibited the production of anti-RNP-Sm antibody cell cultures from MRL-lpr mice. SDS-PAGE analysis of purified S-50 TsF revealed a B16G-reactive band with a molecular weight of 43 kd.
我们之前曾描述过一种单克隆抗体B16G，发现它对T细胞衍生的抑制因子（TsF）具有特异性。已证明B16G可与T抑制细胞、正常或荷瘤小鼠脾脏中的TsF、肿瘤特异性或半抗原特异性T细胞杂交瘤产生的TsF以及从扁桃体组织中分离出的多克隆全人TsF发生反应。B16G抗体固有的这种泛反应性表明它识别TsF分子的一些共同的、共享的表位。在本研究中，我们使用B16G作为探针，从CBA小鼠（H-2k）中分离出一种产生TsF的T细胞杂交瘤S-50，它对狼疮相关抗原RNP-Sm具有特异性。该TsF特异性结合RNP-Sm，并抑制MRL-lpr小鼠抗RNP-Sm抗体细胞培养物的产生。对纯化的S-50 TsF进行SDS-PAGE分析，显示出一条分子量为43 kd的B16G反应带。
