Steele J K, Stammers A T, Levy J G, Waterfield J D
Cell Immunol. 1986 Oct 15;102(2):386-94. doi: 10.1016/0008-8749(86)90432-6.
We have previously described a monoclonal antibody, B16G, which has been found to be specific for T-cell derived suppressor factors (TsF). B16G has been shown to react with T-suppressor cells, TsF in the spleens of normal or tumor-bearing mice, the TsF produced by a tumor-specific T-cell hybridoma, and with polyclonal whole human TsF isolated from tonsilar tissue. This panreactivity inherent to the B16G MAb has made it clear that it recognizes some common, shared epitope of the TsF molecule. In this study we have used B16G as a probe to isolate TsF from the spleens of MRL-lpr mice and compare the activity with these factors isolated from the spleens of an MHC compatible nonautoimmune strain, CBA. We find that equivalent quantities of functional TsF are isolable from both strains and thus, it can be concluded that the associated oligoclonal B-cell activation characteristic of MRL-lpr mice is not due to a polyclonal T-suppressor cell deficit, nor to the ability of TsC in these mice to produce soluble, functional TsFs. The molecular and biochemical characteristics of these TsFs are discussed.
我们之前描述过一种单克隆抗体B16G，已发现它对T细胞衍生的抑制因子（TsF）具有特异性。已证明B16G可与正常或荷瘤小鼠脾脏中的T抑制细胞、TsF、肿瘤特异性T细胞杂交瘤产生的TsF以及从扁桃体组织中分离的多克隆全人TsF发生反应。B16G单克隆抗体固有的这种泛反应性表明它识别TsF分子的一些共同的、共享的表位。在本研究中，我们使用B16G作为探针从MRL-lpr小鼠脾脏中分离TsF，并将其活性与从MHC相容的非自身免疫品系CBA脾脏中分离的这些因子进行比较。我们发现从两个品系中均可分离出等量的功能性TsF，因此，可以得出结论，MRL-lpr小鼠相关的寡克隆B细胞活化特征不是由于多克隆T抑制细胞缺陷，也不是由于这些小鼠中的TsC产生可溶性功能性TsF的能力。本文讨论了这些TsF的分子和生化特征。
