Bright G M, Rust P F
Department of Pediatrics, Medical University of South Carolina, Charleston 29425.
Autoimmunity. 1989;3(4):281-7. doi: 10.3109/08916938908997098.
The source of variability in the outcome of islet cell antibody (ICA) determinations due to pancreas and serum were independently assessed with a quantitative assay. The effect of choice of pancreatic substrate and serum specimen was assessed by testing for parallelism among twenty-five dilution curves of five sera from diabetic subjects and five pancreata. The five by five format allowed for five tests of pancreatic variability (one serum against five pancreata) and five tests of serum variability (one pancreas against five sera). Analyses of variance and covariance of these dilution curves indicate that test outcome is affected by both the sera (5 tests, p values 10(-6) to 10(-7) and the pancreas (5 tests, p values 10(-3) to 10(-5)). The data support heterogeneity among islet cell antibody isotypes and raise the possibility of diversity of islet cell antigens. Antigenic diversity of pancreatic substrates may interfere with inter- and intra-laboratory comparisons of islet cell antibody determinations. The strong effect of pancreas type on ICA outcome infers possible heterogeneity of the islet cell antigen(s) capable of reacting with human islet cell antibodies.
采用定量检测法独立评估了胰腺和血清导致胰岛细胞抗体（ICA）检测结果变异性的来源。通过检测来自糖尿病患者的五份血清和五个胰腺的二十五条稀释曲线之间的平行性，评估了胰腺底物和血清样本选择的影响。这种5×5的形式允许进行五次胰腺变异性测试（一份血清针对五个胰腺）和五次血清变异性测试（一个胰腺针对五份血清）。对这些稀释曲线的方差分析和协方差分析表明，检测结果受血清（5次测试，p值为10⁻⁶至10⁻⁷）和胰腺（5次测试，p值为10⁻³至10⁻⁵）的影响。数据支持胰岛细胞抗体同种型之间的异质性，并增加了胰岛细胞抗原多样性的可能性。胰腺底物的抗原多样性可能会干扰胰岛细胞抗体检测在实验室间和实验室内的比较。胰腺类型对ICA结果的强烈影响推断出能够与人胰岛细胞抗体发生反应的胰岛细胞抗原可能存在异质性。
