Fossat Nicolas, Tourle Karin, Radziewic Tania, Barratt Kristen, Liebhold Doreen, Studdert Joshua B, Power Melinda, Jones Vanessa, Loebel David A F, Tam Patrick P L
Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia Discipline of Medicine, Sydney Medical School University of Sydney, Sydney, NSW, Australia
Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia.
EMBO Rep. 2014 Aug;15(8):903-10. doi: 10.15252/embr.201438450. Epub 2014 Jun 10.
Cytidine (C) to Uridine (U) RNA editing is a post-transcriptional modification that is accomplished by the deaminase APOBEC1 and its partnership with the RNA-binding protein A1CF. We identify and characterise here a novel RNA-binding protein, RBM47, that interacts with APOBEC1 and A1CF and is expressed in tissues where C to U RNA editing occurs. RBM47 can substitute for A1CF and is necessary and sufficient for APOBEC1-mediated editing in vitro. Editing is further impaired in Rbm47-deficient mutant mice. These findings suggest that RBM47 and APOBEC1 constitute the basic machinery for C to U RNA editing.
胞苷（C）到尿苷（U）的RNA编辑是一种转录后修饰，由脱氨酶APOBEC1及其与RNA结合蛋白A1CF的协同作用完成。我们在此鉴定并表征了一种新型RNA结合蛋白RBM47，它与APOBEC1和A1CF相互作用，并在发生C到U RNA编辑的组织中表达。RBM47可以替代A1CF，并且在体外对于APOBEC1介导的编辑是必需且充分的。在Rbm47缺陷型突变小鼠中，编辑功能进一步受损。这些发现表明，RBM47和APOBEC1构成了C到U RNA编辑的基本机制。
