Li Juan, Ding Xiaoming, Zheng Jiaxin, Liu Dandan, Guo Fei, Liu Hongmin, Zhang Yanbing
School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China; New Drug Research & Development Center, Zhengzhou University, Zhengzhou, China.
J Sep Sci. 2014 Sep;37(17):2439-45. doi: 10.1002/jssc.201400349. Epub 2014 Jul 10.
A sensitive and efficient method was developed for the simultaneous determination of eight synthetic dyes (Chrysoidin, Auramine O, Sudan(I-IV), Para Red, and Rhodamine B) in bean and meat products using high-performance liquid chromatography with tandem mass spectrometry. A simple extraction procedure using acetonitrile has been applied for the extraction of these dyes from spiked bean and meat samples. Chromatographic separation was achieved on a Waters XTerra C18 column (2.1 × 150 mm, 5 μm) with a multistep gradient elution. Detection and quantification were performed using mass spectrometry in multiple reaction monitoring mode. Linear calibrations were obtained with correlation coefficients R(2) > 0.99. The limits of detection and quantification for the eight dyes were in the ranges of 0.03-0.75 and 0.1-2.0 μg/kg depending on matrices, respectively. The recoveries of these dyes in different food matrices were between 71.2 and 116.9% with relative standard deviations <15.2%, suggesting that the developed method is promising for the accurate quantification of the eight dyes at trace levels in bean and meat products.
建立了一种灵敏高效的方法，采用高效液相色谱-串联质谱法同时测定豆类和肉类产品中的8种合成染料（碱性菊橙、金胺O、苏丹红（I-IV）、对位红和罗丹明B）。采用简单的乙腈萃取程序从添加了染料的豆类和肉类样品中萃取这些染料。在Waters XTerra C18柱（2.1×150 mm，5μm）上进行多步梯度洗脱实现色谱分离。采用多反应监测模式的质谱法进行检测和定量。获得了相关系数R(2)>0.99的线性校准曲线。8种染料的检测限和定量限分别在0.03-0.75μg/kg和0.1-2.0μg/kg范围内，具体取决于基质。这些染料在不同食品基质中的回收率在71.2%至116.9%之间，相对标准偏差<15.2%，表明所建立的方法有望用于准确测定豆类和肉类产品中痕量水平的8种染料。
