Chemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Chemistry Department, Faculty of Science, Jazan University, Jazan 45142, Saudi Arabia.
Molecules. 2020 Oct 6;25(19):4564. doi: 10.3390/molecules25194564.
Lower dye concentrations and the presence of several dyes along with other matrices in environmental samples restrict their determination. Herein, a highly sensitive and rapid ultra-performance tandem mass spectrometric method was developed for simultaneous determination of cationic dyes, namely methylene blue (MB), rhodamine B (RB) and crystal violet (CV), in environmental samples. To preconcentrate environmental samples, solid-phase extraction cartridges were developed by using hydrogen peroxide modified pistachio shell biomass (MPSB). The surface morphological and chemical functionalities of MPSB were well characterized. The developed method was validated considering different validation parameters. In terms of accuracy and precision, the %RSD for all three dyes at all four concentration points was found to be between 1.26 and 2.76, while the accuracy reported in terms of the recovery was found to be 98.02%-101.70%. The recovery was found to be in the range of 98.11% to 99.55%. The real sample analysis shows that MB, RB, and CV were found in the ranges of 0.39-5.56, 0.32-1.92 and 0.27-4.36 μg/mL, respectively.
较低的染料浓度以及环境样品中存在的多种染料和其他基质限制了它们的测定。本文建立了一种高灵敏度、快速的超高效串联质谱法，用于同时测定环境样品中的阳离子染料，即亚甲蓝（MB）、罗丹明 B（RB）和结晶紫（CV）。为了预浓缩环境样品，采用过氧化氢改性的开心果壳生物质（MPSB）制备了固相萃取小柱。对 MPSB 的表面形态和化学官能团进行了很好的表征。考虑到不同的验证参数对开发的方法进行了验证。就准确度和精密度而言，在所有四个浓度点，所有三种染料的%RSD 均在 1.26%至 2.76%之间，而以回收率表示的准确度在 98.02%至 101.70%之间。回收率在 98.11%至 99.55%之间。实际样品分析表明，MB、RB 和 CV 的浓度范围分别为 0.39-5.56μg/mL、0.32-1.92μg/mL 和 0.27-4.36μg/mL。
