Pesoa S A, Vullo C M, Onetti C M, Riera C M
Catedra de Inmunologia y Serologia, Facultad de Ciencas Quimicas, Universidad Nacional de Cordoba, Argentina.
Autoimmunity. 1989;4(3):171-9. doi: 10.3109/08916938909003047.
The distribution of frequencies of HLA-DR alloantigens in HLA-DR4 negative subjects was determined in patients with Rheumatoid arthritis (RA) and normal individuals. An increased incidence of HLA-DR1 alloantigen in DR4 negative RA patients (45.9%) compared with DR4 negative healthy controls (23.6%) was found. The difference became significant when the incidence of DR1 was compared between patients with severe disease stages (III-IV) (75%) in contrast to 32% of incidence in patients of the milder stages (I-II) (p less than 0.05). Using Enzyme Linked Immunosorbent Assay we have determined the incidence of serum antibodies to native bovine type I and type II collagens and proteoglycans in patients with RA. Presence of serum antibodies to native type I collagen was detected in 59% of patients with RA, 60% of sera exhibited reactivity to type II collagen and 12% had antibodies to proteoglycans. There was no correlation between the presence of antibodies to type I and II collagens and disease stages, however, the incidence of serum antibodies to proteoglycans was increased in severe disease stages. On the other hand, the presence of high levels of antibodies to type I collagen was associated to HLA-DR1 antigen, (p less than 0.05).
在类风湿性关节炎（RA）患者和正常个体中，确定了HLA - DR4阴性受试者中HLA - DR同种异体抗原的频率分布。发现与DR4阴性健康对照（23.6%）相比，DR4阴性RA患者中HLA - DR1同种异体抗原的发生率增加（45.9%）。当比较疾病严重阶段（III - IV）患者中DR1的发生率（75%）与较轻阶段（I - II）患者中32%的发生率时，差异变得显著（p小于0.05）。我们使用酶联免疫吸附测定法确定了RA患者血清中针对天然牛I型和II型胶原蛋白以及蛋白聚糖的抗体发生率。在59%的RA患者中检测到针对天然I型胶原蛋白的血清抗体，60%的血清对II型胶原蛋白有反应，12%的患者有针对蛋白聚糖的抗体。I型和II型胶原蛋白抗体的存在与疾病阶段之间没有相关性，然而，在疾病严重阶段，血清中针对蛋白聚糖的抗体发生率增加。另一方面，高水平的I型胶原蛋白抗体的存在与HLA - DR1抗原相关（p小于0.05）。
