Clarke A E, Anderson M A, Atkinson A, Bacic A, Ebert P R, Jahnen W, Lush W M, Mau S L, Woodward J R
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Plant Mol Biol. 1989 Sep;13(3):267-71. doi: 10.1007/BF00025314.
A summary of recent work on molecular aspects of self-incompatibility in Nicotiana alata is presented. The amino acid sequences of style proteins corresponding to different S-alleles of N. alata have a high level of homology in some regions and are variable in other regions. The regions of homology include N-terminal sequences as well as most of the glycosylation sites and cysteine residues. The glycosyl substituents may consist of a number of 'glycoforms'. The isolated style S-glycoproteins inhibit in vitro growth of pollen tubes. The S-glycoproteins tested inhibited the growth of pollen of several S-genotypes, and there was some specificity in the interaction. Heat treatment of the isolated S-glycoproteins dramatically increased their activity as inhibitors of pollen tube growth, although the specificity in the interaction was lost. The nature of the S-allele products in pollen is not yet established.
本文综述了烟草自交不亲和分子机制的最新研究进展。与烟草不同S等位基因相对应的花柱蛋白的氨基酸序列在某些区域具有高度同源性，而在其他区域则存在差异。同源区域包括N端序列以及大部分糖基化位点和半胱氨酸残基。糖基取代基可能由多种“糖型”组成。分离得到的花柱S糖蛋白在体外抑制花粉管生长。所测试的S糖蛋白抑制了几种S基因型花粉的生长，并且在相互作用中存在一定的特异性。对分离得到的S糖蛋白进行热处理，虽然失去了相互作用的特异性，但显著提高了它们作为花粉管生长抑制剂的活性。花粉中S等位基因产物的性质尚未明确。
