Department of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UK.
Department of Clinical Science Services, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK.
FEBS Open Bio. 2014 Mar 3;4:266-75. doi: 10.1016/j.fob.2014.02.008. eCollection 2014.
Spermatozoa and lentiviruses are two of nature's most efficient gene delivery vehicles. Both can be genetically modified and used independently for the generation of transgenic animals or gene transfer/therapy of inherited disorders. Here we show that mature spermatozoa can be directly transduced with various pseudotyped lentiviral vectors and used in in vitro fertilisation studies. Lentiviral vectors encoding Green Fluorescent Protein (GFP) were shown to be efficiently processed and expressed in sperm. When these transduced sperm were used in in vitro fertilisation studies, GFP expression was observed in arising blastocysts. This simple technique of directly transducing spermatozoa has potential to be a powerful tool for the study of early and pre-implantation development and could be used as a technique in transgenic development and vertical viral transmission studies.
精子和慢病毒是自然界中两种最有效的基因传递载体。这两种都可以进行基因修饰，并独立用于转基因动物的产生或遗传性疾病的基因转移/治疗。在这里，我们表明，各种假型慢病毒载体可以直接转导成熟精子，并用于体外受精研究。已显示携带绿色荧光蛋白 (GFP) 的慢病毒载体在精子中被有效地加工和表达。当这些转导的精子用于体外受精研究时，在出现的胚泡中观察到 GFP 表达。这种直接转导精子的简单技术有可能成为研究早期和植入前发育的有力工具，并且可以用作转基因发展和垂直病毒传播研究的技术。
