Chikezie Paul C, Uwakwe Augustine A
Department of Biochemistry, Imo state university, Owerri 460222, Nigeria.
Department of Biochemistry, University of Port Harcourt, Port Harcourt 460222, Nigeria.
J Diabetes Metab Disord. 2014 Apr 22;13:50. doi: 10.1186/2251-6581-13-50. eCollection 2014.
The present study sought to investigate erythrocyte glutathione S-transferases (GST), NADH-Methaemoglobin reductase (NADH-MR) and Na(+)/K(+)-ATPase activities of hypoglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa as agent of glycemic control.
Hyperglycemia was induced by a single intra-peritoneal injection of 0.1 mol/L alloxan monohydrate in phosphate buffer saline (PBS) solution (pH = 7.4); dosage = 140 mg/kg. At the end of the experimental time (t = 76 h), erythrocyte GST, NADH-MR and Na(+)/K(+)-ATPase activities as well as serum fasting blood sugar (FBS) levels were measured by spectrophotometric methods.
Serum FBS levels of control/normal (C/N) rats ranged between 72.93 ± 0.82-95.12 ± 0.92 mg/dL, whereas experimental rats without glycemic control gave: 249.41 ± 1.03-256.11 ± 1.23 mg/dL. Hyperglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa exhibited comparative reduced serum levels of FBS alongside with erythrocyte GST, NADH-MR and Na(+)/K(+)-ATPase activities. The average relative activities of the three enzymes and corresponding order of enzyme activity in hyperglycemic rats treated with ethanol/water (1:2 v/v) extract of A. sativa was: NADH-MR = 60.99% > GST = 47.81% > Na(+)/K(+)-ATPase = 46.81%. In the same order, relative activities of the three enzymes in rats without glycemic control were: NADH-MR = 49.65% > GST = 23.69% > Na(+)/K(+)-ATPase = 17.02%.
Erythrocyte GST, NADH-MR and Na(+)/K(+)-ATPase activities gave insights into the pathophysiology of diabetic state and served as biomarkers for ascertaining therapeutic control in Type 1 diabetes mellitus.
本研究旨在调查用紫花苜蓿乙醇/水（1:2，v/v）提取物作为血糖控制剂治疗的低血糖大鼠的红细胞谷胱甘肽S-转移酶（GST）、NADH-高铁血红蛋白还原酶（NADH-MR）和Na(+)/K(+)-ATP酶活性。
通过在磷酸盐缓冲盐水（PBS）溶液（pH = 7.4）中单次腹腔注射0.1 mol/L一水合四氧嘧啶诱导高血糖；剂量 = 140 mg/kg。在实验时间结束时（t = 76小时），通过分光光度法测量红细胞GST、NADH-MR和Na(+)/K(+)-ATP酶活性以及血清空腹血糖（FBS）水平。
对照/正常（C/N）大鼠的血清FBS水平在72.93±0.82 - 95.12±0.92 mg/dL之间，而未进行血糖控制的实验大鼠的血清FBS水平为：249.41±1.03 - 256.11±1.23 mg/dL。用紫花苜蓿乙醇/水（1:2，v/v）提取物治疗的高血糖大鼠的血清FBS水平以及红细胞GST、NADH-MR和Na(+)/K(+)-ATP酶活性相对降低。在用紫花苜蓿乙醇/水（1:2，v/v）提取物治疗的高血糖大鼠中，这三种酶的平均相对活性及酶活性的相应顺序为：NADH-MR = 60.99% > GST = 四、结论红细胞GST、NADH-MR和Na(+)/K(+)-ATP酶活性有助于深入了解糖尿病状态的病理生理学，并可作为确定1型糖尿病治疗控制情况的生物标志物。
47.81% > Na(+)/K(+)-ATPase = 46.81%。按相同顺序，未进行血糖控制的大鼠中这三种酶的相对活性为：NADH-MR = 49.65% > GST = 23.69% > Na(+)/K(+)-ATPase = 17.02%。
红细胞GST、NADH-MR和Na(+)/K(+)-ATP酶活性有助于深入了解糖尿病状态的病理生理学，并可作为确定1型糖尿病治疗控制情况的生物标志物。
