Murugan Pidaran, Pari Leelavinothan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India.
J Ethnopharmacol. 2007 Sep 25;113(3):479-86. doi: 10.1016/j.jep.2007.07.004. Epub 2007 Jul 10.
Curcuma longa (Zingiberaceae) has been used traditionally as antidiabetic and has been proven scientifically to possess high antioxidant activity and anticancer properties. The active components of Curcuma longa such as curcumin and tetrahydrocurcumin (THC), a major colourless metabolite of curcumin also possesses antidiabetic, antiinflammatory and antioxidant activity. In the present study the effect of THC and curcumin on erythrocyte membrane bound enzymes and antioxidants activity in streptozotocin (STZ) and nicotinamide induced type 2 diabetic model was investigated. Oral administration of THC at 80 mg/kg body weight to diabetic rats for 45 days. The effect of THC and curcumin on glucose, insulin, haemoglobin, glycosylated haemoglobin, thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), catalase (CAT), glutathione peroxide (Gpx), glutathione-S-transferase (GST), reduced glutathione (GSH) and membrane bound enzymes were studied. The effect of THC was compared with curcumin. The levels of blood glucose, glycosylated haemoglobin, erythrocyte TBARS, were increased significantly whereas the level of plasma insulin and haemoglobin, erythrocyte antioxidants (SOD, CAT, GPx, GST and GSH), membrane bound total ATPase, Na(+)/K(+)-ATPase, Ca(2+)-ATPase, Mg(2+)-ATPase were decreased significantly in diabetic rats. Administration of THC and curcumin to diabetic rats showed decreased level of blood glucose, glycosylated haemoglobin and erythrocyte TBARS. In addition the levels of plasma insulin, haemoglobin, erythrocyte antioxidants and the activities of membrane bound enzymes also were increased in THC and curcumin treated diabetic rats. These biochemical observations were supplemented by histopathological examination of pancreas section. The present study indicates that the THC possesses a significant beneficial effect on erythrocyte membrane bound enzymes and antioxidants defense in addition to its antidiabetic effect.
姜黄（姜科）传统上一直被用作抗糖尿病药物，并且已被科学证明具有高抗氧化活性和抗癌特性。姜黄的活性成分如姜黄素和四氢姜黄素（THC），姜黄素的一种主要无色代谢产物也具有抗糖尿病、抗炎和抗氧化活性。在本研究中，研究了THC和姜黄素对链脲佐菌素（STZ）和烟酰胺诱导的2型糖尿病模型中红细胞膜结合酶和抗氧化剂活性的影响。以80mg/kg体重的剂量给糖尿病大鼠口服THC，持续45天。研究了THC和姜黄素对血糖、胰岛素、血红蛋白、糖化血红蛋白、硫代巴比妥酸反应性物质（TBARS）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（Gpx）、谷胱甘肽-S-转移酶（GST）、还原型谷胱甘肽（GSH）和膜结合酶的影响。将THC的作用与姜黄素进行了比较。糖尿病大鼠的血糖、糖化血红蛋白、红细胞TBARS水平显著升高，而血浆胰岛素和血红蛋白水平、红细胞抗氧化剂（SOD、CAT、GPx、GST和GSH）、膜结合总ATP酶、Na(+)/K(+)-ATP酶、Ca(2+)-ATP酶、Mg(2+)-ATP酶水平显著降低。给糖尿病大鼠施用THC和姜黄素后，血糖、糖化血红蛋白和红细胞TBARS水平降低。此外，在THC和姜黄素治疗的糖尿病大鼠中，血浆胰岛素、血红蛋白、红细胞抗氧化剂水平以及膜结合酶的活性也有所增加。这些生化观察结果得到了胰腺切片组织病理学检查的补充。本研究表明，THC除了具有抗糖尿病作用外，对红细胞膜结合酶和抗氧化防御也具有显著的有益作用。
