Ogata N, Yonekawa Y, Taki W, Kannagi R, Murachi T, Hamakubo T, Kikuchi H
Department of Neurosurgery, Kyoto University Faculty of Medicine, Japan.
J Neurosurg. 1989 Jan;70(1):103-7. doi: 10.3171/jns.1989.70.1.0103.
Degradation of neurofilament (NF) triplet proteins: NF200 (molecular weight (MW) 200,000), NF150 (MW 150,000), and NF68 (MW 68,000) as well as of other cytoskeletal proteins in the rat brain during ischemia was investigated. Sodium dodecyl sulfate-gel electrophoresis and immunoblot methods with anti-NF200 antibody were used for the study. Selective degradation of NF200 and NF150 was observed during the initial 10 to 15 minutes of ischemia. The degradation was demonstrated both in permanent ischemia caused by decapitation and in transient ischemia induced by four-vessel occlusion followed by reperfusion after 30 minutes of occlusion (modified Pulsinelli method). The degradation suggests that the activation of a protease occurs in the first 15 minutes of cerebral ischemia, which is the earliest irreversible neuronal change ever to be reported.
研究了大鼠脑缺血期间神经丝（NF）三联体蛋白（分子量200,000的NF200、分子量150,000的NF150和分子量68,000的NF68）以及其他细胞骨架蛋白的降解情况。采用十二烷基硫酸钠凝胶电泳和抗NF200抗体免疫印迹法进行研究。在缺血最初的10至15分钟内观察到NF200和NF150的选择性降解。断头所致的永久性缺血以及四血管闭塞30分钟后再灌注（改良Pulsinelli法）所致的短暂性缺血中均证实了这种降解。这种降解表明在脑缺血的最初15分钟内发生了蛋白酶激活，这是迄今报道的最早的不可逆神经元变化。
