关于大肠杆菌HF4714的抑制性质。
On the nature of suppression by Escherichia coli HF4714.
作者信息
Chambers R W
机构信息
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
出版信息
Mutat Res. 1989 Jan;210(1):207-9. doi: 10.1016/0027-5107(89)90060-2.
The growth of 2 well characterized bacteriophage phi X174 mutants, phi XGam3 and phi XGms3 (TCG)Ser, was examined on 3 suppressor strains of Escherichia coli: CQ3 Su1, which inserts serine at amber codons; HF4714, a well known amber suppressor that has been used as the permissive strain for a number of phi X mutants; and CQ2 Su3, which inserts tyrosine. The data demonstrate HF4714 inserts glutamine and is Su2, not Su1 as has been reported in several recent papers.
在3株大肠杆菌抑制菌株上检测了2种特性明确的噬菌体φX174突变体φXGam3和φXGms3(TCG)Ser的生长情况，这3株菌株分别是：CQ3 Su1，它在琥珀密码子处插入丝氨酸；HF4714，一种著名的琥珀抑制菌株，已被用作多种φX突变体的允许菌株；以及CQ2 Su3，它插入酪氨酸。数据表明，HF4714插入谷氨酰胺，是Su2，而不是最近几篇论文中报道的Su1。
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