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Direct measurement of absolute suppressor efficiency.

作者信息

Nene V, Glass R E

出版信息

Biosci Rep. 1981 Jul;1(7):571-4. doi: 10.1007/BF01116306.

DOI:10.1007/BF01116306
PMID:7028161
Abstract

We have devised a system for measuring the degree of translational readthrough past a nonsense mutation which is based upon the quantitation of the two translation products, the suppressed polypeptide and the nonsense fragment. The absolute efficiency of four different amber suppressors (Su1, Su2, Su3, and Su7) has been determined at two unique amber sites in the structural gene for the beta subunit of Escherichia coli RNA polymerase.

摘要

相似文献

1
Direct measurement of absolute suppressor efficiency.
Biosci Rep. 1981 Jul;1(7):571-4. doi: 10.1007/BF01116306.
2
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引用本文的文献

1
Analysis of btuB receptor function by use of nonsense suppression.利用无义抑制分析btuB受体功能。
J Bacteriol. 1982 Sep;151(3):1591-4. doi: 10.1128/jb.151.3.1591-1594.1982.
2
Genetic studies on the beta subunit of Escherichia coli RNA polymerase. II. Evidence that large N-terminal amber fragments of the beta subunit interfere with RNA polymerase function.大肠杆菌RNA聚合酶β亚基的遗传学研究。II. β亚基的大N端琥珀色片段干扰RNA聚合酶功能的证据。
Mol Gen Genet. 1982;188(3):405-9. doi: 10.1007/BF00330041.
3
Genetic studies on the beta subunit of Escherichia coli RNA polymerase. I. The effect of known, single amino acid substitutions in an essential protein.
大肠杆菌RNA聚合酶β亚基的遗传学研究。I. 一种必需蛋白质中已知单氨基酸取代的影响。
Mol Gen Genet. 1982;188(3):399-404. doi: 10.1007/BF00330040.
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Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VI. A redundant region in the beta polypeptide.大肠杆菌RNA聚合酶β亚基的遗传学研究。VI. β多肽中的一个冗余区域。
Mol Gen Genet. 1984;196(1):64-7. doi: 10.1007/BF00334093.
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Genetic studies on the beta subunit of Escherichia coli RNA polymerase. IV. Structure-function correlates.大肠杆菌RNA聚合酶β亚基的遗传学研究。IV. 结构-功能相关性
Mol Gen Genet. 1984;194(1-2):166-72. doi: 10.1007/BF00383512.
6
Autogenous posttranscriptional regulation of RNA polymerase beta and beta' subunit synthesis in Escherichia coli.大肠杆菌中RNA聚合酶β和β'亚基合成的自体转录后调控
J Bacteriol. 1985 Feb;161(2):803-6. doi: 10.1128/jb.161.2.803-806.1985.