Zhang Xi-Feng, Zhang Teng, Han Zhe, Liu Jing-Cai, Liu Yu-Ping, Ma Jun-Yu, Li Lan, Shen Wei
Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
Reprod Fertil Dev. 2015 Nov;27(8):1213-21. doi: 10.1071/RD14113.
Diethylhexyl phthalate (DEHP) is a widely used industrial additive for increasing plastic flexibility. It disrupts the physiological functions of endogenous hormones and induces abnormal development of mammals. The objectives of the present study were to evaluate the effects of DEHP exposure on ovarian development of pregnant mice and whether the effects are inheritable. We found that the synthesis of oestradiol in pregnant mice after DEHP exposure was significantly decreased, and that the first meiotic progression of female fetal germ cells was delayed. Furthermore, the DNA methylation level of Stra8 was increased and the expression levels of Stra8 were significantly decreased. An accelerated rate of follicle recruitment in F1 mice was responsible for the depletion of the primordial-follicle pool. Maternal DEHP exposure also significantly accelerated the recruitment of primordial follicles in F2 mice. In conclusion, our results indicated that maternal DEHP exposure induced ovarian development deficiency, which was transgenerational in mice.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种广泛使用的用于增加塑料柔韧性的工业添加剂。它会干扰内源性激素的生理功能，并诱导哺乳动物发育异常。本研究的目的是评估DEHP暴露对怀孕小鼠卵巢发育的影响以及这些影响是否具有遗传性。我们发现，DEHP暴露后怀孕小鼠体内雌二醇的合成显著减少，并且雌性胎儿生殖细胞的第一次减数分裂进程延迟。此外，Stra8的DNA甲基化水平升高，而Stra8的表达水平显著降低。F1代小鼠卵泡募集速率加快导致原始卵泡池减少。母体DEHP暴露也显著加速了F2代小鼠原始卵泡的募集。总之，我们的结果表明，母体DEHP暴露会诱导卵巢发育缺陷，且这种缺陷在小鼠中具有跨代效应。
