Tsukada Hideo, Nishiyama Shingo, Ohba Hiroyuki, Kanazawa Masakatsu, Kakiuchi Takeharu, Harada Norihiro
Central Research Laboratory, Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamakita, Shizuoka, 434-8601, Japan,
Eur J Nucl Med Mol Imaging. 2014 Nov;41(11):2127-36. doi: 10.1007/s00259-014-2821-8. Epub 2014 Jun 12.
The aim of the present study was to compare amyloid-β (Aβ) deposition, translocator protein (TSPO) activity, regional cerebral metabolic rate of glucose (rCMRglc), and mitochondrial complex I (MC-I) activity in the brain of aged monkeys.
PET scans with (11)C-PIB (Aβ), (18)F-BCPP-EF (MC-I), (11)C-DPA-713 (TSPO), and (18)F-FDG (rCMRglc) were performed in aged monkeys (Macaca mulatta) in the conscious state and under isoflurane anaesthesia. (11)C-PIB binding to Aβ and (11)C-DPA-713 binding to TSPO were evaluated in terms of standard uptake values (SUV). The total volume of distribution (V T) of (18)F-BCPP-EF and rCMRglc with (18)F-FDG were calculated using arterial blood sampling.
Isoflurane did not affect MC-I activity measured in terms of (18)F-BCPP-EF uptake in living brain. There was a significant negative correlation between (18)F-BCPP-EF binding (V T) and (11)C-PIB uptake (SUVR), and there was a significant positive correlation between (11)C-DPA-713 uptake (SUV) and (11)C-PIB uptake. In contrast, there was no significant correlation between rCMRglc ratio and (11)C-PIB uptake.
(18)F-BCPP-EF could be a potential PET probe for quantitative imaging of impaired MC-I activity that is correlated with Aβ deposition in the living brain.
本研究旨在比较老年猴大脑中β淀粉样蛋白（Aβ）沉积、转位蛋白（TSPO）活性、局部脑葡萄糖代谢率（rCMRglc）和线粒体复合物I（MC-I）活性。
对清醒状态和异氟烷麻醉下的老年恒河猴（猕猴）进行正电子发射断层扫描（PET），分别使用（11）C-PIB（用于检测Aβ）、（18）F-BCPP-EF（用于检测MC-I）、（11）C-DPA-713（用于检测TSPO）和（18）F-FDG（用于检测rCMRglc）。根据标准摄取值（SUV）评估（11）C-PIB与Aβ的结合以及（11）C-DPA-713与TSPO的结合。通过动脉血采样计算（18）F-BCPP-EF的分布总体积（VT）和（18）F-FDG的rCMRglc。
异氟烷不影响活体大脑中通过（18）F-BCPP-EF摄取测量的MC-I活性。（18）F-BCPP-EF结合（VT）与（11）C-PIB摄取（SUVR）之间存在显著负相关，（11）C-DPA-713摄取（SUV）与（11）C-PIB摄取之间存在显著正相关。相比之下，rCMRglc比值与（11）C-PIB摄取之间无显著相关性。
（18）F-BCPP-EF可能是一种潜在的PET探针，用于定量成像与活体大脑中Aβ沉积相关的受损MC-I活性。
