Mao Xun, Gurung Anant, Xu Hui, Baloda Meenu, He Yuqing, Liu Guodong
Department of Chemistry and Biochemistry, North Dakota State University.
Anal Sci. 2014;30(6):637-42. doi: 10.2116/analsci.30.637.
In this work, we present a simple and fast approach for simultaneous detection of nucleic acid and protein using gold nanoparticles (GNPs) and a lateral flow device (LFD). Sandwich-type immunoreactions and DNA hybridizations were performed simultaneously on the LFD by using DNA- and antibody-functionalized GNPs. The captured GNPs, due to the DNA hybridization and immunoreaction events on the LFD, produced characteristic red bands that could be used for the qualitative detections of DNA and/or protein. The proof of principle was demonstrated by using 60-mer DNA and rabbit IgG (R-IgG) model targets. The LFD was capable of detecting a minimum of 0.5 nM target DNA and 2 ng mL(-1) IgG simultaneously in 15 min. The proposed LFD shows great promise for in-field and point-of-care testing of disease-related circulating nucleic acid and protein biomarkers in biological fluids.
在本研究中，我们展示了一种使用金纳米颗粒（GNPs）和侧向流动装置（LFD）同时检测核酸和蛋白质的简单快速方法。通过使用DNA和抗体功能化的GNPs，在LFD上同时进行夹心型免疫反应和DNA杂交。由于LFD上的DNA杂交和免疫反应事件，捕获的GNPs产生特征性红色条带，可用于DNA和/或蛋白质的定性检测。使用60聚体DNA和兔免疫球蛋白G（R-IgG）模型靶标证明了该原理。该LFD能够在15分钟内同时检测低至0.5 nM的靶标DNA和2 ng mL(-1)的IgG。所提出的LFD在生物流体中疾病相关循环核酸和蛋白质生物标志物的现场和即时检测方面显示出巨大潜力。
