Zhang Hongying, Jia Hongfang, Liu Guoshun, Yang Shengnan, Zhang Songtao, Yang Yongxia, Yang Peipei, Cui Hong
Key Laboratory for Cultivation of Tobacco Industry, College of Tobacco Science, Henan Agricultural University, Zhengzhou, 450002, China.
Mol Biol Rep. 2014 Sep;41(9):5701-9. doi: 10.1007/s11033-014-3440-y. Epub 2014 Jun 12.
SnRK2 is a plant-specific protein kinase family involved in abiotic stress signalling. In this study, NtSnRK2.1, NtSnRK2.2, and NtSnRK2.3, were cloned from tobacco by in silico cloning and reverse transcription PCR. The three protein kinases were classed into subfamily II of the SnRK2 family using a phylogenetic tree and C-terminus analysis. Subcellular localization revealed NtSnRK2s in the nuclear and cytoplasmic compartments. Dynamic expression of NtSnRK2s in tobacco plants that were exposed to drought, salt, or cold stressors were characterised using quantitative real-time PCR. It was revealed that the three genes showed similar patterns of transcription under abiotic stress responses; there was evidence NtSnRK2s participated in abscisic acid-dependent signalling pathways. NtSnRK2.1-3 responded much faster to drought and salt than to cold stress. To investigate the role of NtSnRK2s under abiotic stresses, NtSnRK2.1 gene was over-expressed in tobacco. A stress tolerance assay showed that tobacco plants that over-expressed NtSnRK2.1 plants had greater salt tolerance. The results indicate that NtSnRK2s are involved in abiotic stress response pathways.
SnRK2是一个参与非生物胁迫信号传导的植物特异性蛋白激酶家族。在本研究中，通过电子克隆和逆转录PCR从烟草中克隆了NtSnRK2.1、NtSnRK2.2和NtSnRK2.3。利用系统发育树和C末端分析将这三种蛋白激酶归类为SnRK2家族的II亚家族。亚细胞定位显示NtSnRK2s存在于细胞核和细胞质区室中。使用定量实时PCR对暴露于干旱、盐或冷胁迫的烟草植株中NtSnRK2s的动态表达进行了表征。结果表明，这三个基因在非生物胁迫反应下表现出相似的转录模式；有证据表明NtSnRK2s参与了脱落酸依赖的信号通路。NtSnRK2.1-3对干旱和盐的反应比对冷胁迫的反应快得多。为了研究NtSnRK2s在非生物胁迫下的作用，在烟草中过表达了NtSnRK2.1基因。胁迫耐受性试验表明，过表达NtSnRK2.1的烟草植株具有更高的耐盐性。结果表明，NtSnRK2s参与非生物胁迫反应途径。
