Andersson A, Korsgren O, Jansson L
Department of Medical Cell Biology, Uppsala University, Sweden.
Diabetes. 1989 Jan;38 Suppl 1:192-5. doi: 10.2337/diab.38.1.s192.
Isolated pancreatic islets were implanted intraportally into normoglycemic or alloxan-induced diabetic C57BL/6 mice. Each animal was grafted with 200-250 syngeneic islets. Four, 8, 14, or 56 days after the transplantation, 2 to 3 x 10(5) microspheres (10 micron diam) were injected into either the portal vein or the abdominal aorta of anesthetized animals. The presence of microspheres within the islet grafts was determined in stained sections of the livers. In general, the number of microspheres in the liver sections was greater than 10 times higher in mice given microspheres via the portal route. When the microspheres were administered via the arterial system 2 wk after transplantation, approximately 20-30% of the islet sections contained microspheres; only occasional spheres were found in the islets after intraportal administration. This was the case even when as many as 56 days had elapsed. Some but not all mice investigated 4 and 8 days after transplantation had islets that contained microspheres. The intraportally implanted islets in hyperglycemic animals had decreased revascularization compared with normoglycemic mice 2 wk after implantation. The results indicate that intraportally implanted syngeneic pancreatic islets become revascularized mainly from the hepatic artery and that this process starts as early as during the 1st wk postimplantation. Furthermore, hyperglycemia seems to influence this process.
将分离的胰岛经门静脉植入血糖正常或经四氧嘧啶诱导的糖尿病C57BL/6小鼠体内。每只动物移植200 - 250个同基因胰岛。移植后4、8、14或56天，将2至3×10(5)个微球（直径10微米）注入麻醉动物的门静脉或腹主动脉。通过肝脏染色切片确定胰岛移植物中微球的存在情况。一般来说，经门静脉途径给予微球的小鼠肝脏切片中的微球数量比经动脉途径给予微球的小鼠高出10倍以上。移植2周后经动脉系统给予微球时，约20 - 30%的胰岛切片含有微球；经门静脉给药后，胰岛中仅偶尔发现微球。即使在56天后也是如此。移植后4天和8天进行研究的部分但并非所有小鼠的胰岛含有微球。与血糖正常的小鼠相比，高血糖动物经门静脉植入的胰岛在植入2周后血管再生减少。结果表明，经门静脉植入的同基因胰岛主要从肝动脉进行血管再生，且这一过程在植入后第1周就开始了。此外，高血糖似乎会影响这一过程。
