Hu Weigang, Zhang Qi, Li Dingyao, Cheng Gang, Mu Jing, Wu Qingbai, Niu Fujun, An Lizhe, Feng Huyuan
Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.
J Basic Microbiol. 2014 Dec;54(12):1331-41. doi: 10.1002/jobm.201400232. Epub 2014 Jun 12.
While a vast number of studies have addressed the prokaryotic diversity in permafrost, characterized by subzero temperatures, low water activity, and extremely low rates of nutrient and metabolite transfer, fungal patterns have received surprisingly limited attention. Here, the fungal diversity and community structure were investigated by culture-dependent technique combined with cloning-restriction fragment length polymorphism (RFLP) analysis of sediments in a 10-m-long permafrost core from the Qinghai-Tibet Plateau of China. A total of 62 fungal phylotypes related to 10 distinct classes representing three phyla were recovered from 5031 clones generated in 13 environmental gene libraries. A large proportion of the phylotypes (25/62) that were distantly related to described fungal species appeared to be novel diversity. Ascomycota was the predominant group of fungi, with respect to both clone and phylotype number. Our results suggested there was the existence of cosmopolitan psychrophilic or psychrotolerant fungi in permafrost sediments, the community composition of fungi varied with increasing depth, while these communities largely distributed according to core layers.
虽然大量研究探讨了多年冻土中的原核生物多样性，其特征为温度低于零度、水分活性低以及营养物质和代谢物转移速率极低，但真菌模式却出人意料地受到了有限关注。在此，通过依赖培养的技术结合对来自中国青藏高原一个10米长的多年冻土岩芯沉积物的克隆-限制性片段长度多态性（RFLP）分析，研究了真菌多样性和群落结构。从13个环境基因文库中产生的5031个克隆中，共回收了62种与代表三个门的10个不同纲相关的真菌系统型。很大一部分与已描述真菌物种关系较远的系统型（25/62）似乎是新的多样性。就克隆数和系统型数量而言，子囊菌门是真菌的主要类群。我们的结果表明，多年冻土沉积物中存在世界性嗜冷或耐冷真菌，真菌群落组成随深度增加而变化，而这些群落主要根据岩芯层分布。
