Cheng Zhichao, Wu Song, Du Jun, Liu Yongzhi, Sui Xin, Yang Libin
Key Laboratory of Biodiversity, Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences, Harbin 150040, China.
Science and Technology Innovation Center, Institute of Scientific and Technical Information of Heilongjiang Province, Harbin 150028, China.
Microorganisms. 2023 Jul 19;11(7):1836. doi: 10.3390/microorganisms11071836.
Forest fires are an important disturbance factor in forest ecosystems, and obviously change the soil environment. Arbuscular mycorrhizal fungi, as a medium and bridge between vegetation and soil, play a crucial role in mediating plant nutrient uptake and regulating the productivity, stability, and succession of vegetation-soil systems. To investigate the effects of forest fires on the community structure and diversity of arbuscular mycorrhizal fungi in cold-temperate forests, we collected soils from light, moderate, and heavy fire disturbance forests and a natural forest as a control forest in Greater Khingan forests, in the northeast of China. The community structure and diversity of arbuscular mycorrhizal fungi was sequenced using Illumina MiSeq technology and we analyzed the correlation with the soil physicochemical characteristics. The results showed that the contents of microbial biomass content (MBC), moisture content (MC), total nitrogen (TN), and available phosphors (AP) increased significantly ( < 0.05) with increasing fire intensity (from Light to heavy fire), but available potassium (AK) decreased significantly ( < 0.05). These changes were not significant. A total of 14,554 valid sequences from all sequences were classified into 66 ASVs that belonged into one phylum, one order, four families, and four genera. The genera included , , , and , and was the dominant genus (the genera with the five most relative abundances) in the control and heavy-fire forests. Non-metric multidimensional scaling (NMDS) analysis showed that forest fires significantly affected the community structure of arbuscular mycorrhizal fungi ( < 0.01). Redundancy analysis (RDA) showed that MBC, SOC, and AP contents significantly affected the composition structure and diversity of arbuscular mycorrhizal fungi communities. This study indicated that forest fires affected the composition and diversity of soil arbuscular mycorrhizal fungi communities through changing the soil physicochemical parameters (MBC, SOC, and AP) in cold-temperate forests. The study of soil physicochemical properties and arbuscular mycorrhizal fungi diversity in cold-temperate forests in the Greater Khingan Mountains after forest fires provides a reference basis for the revegetation and reconstruction of fire sites.
森林火灾是森林生态系统中的一个重要干扰因素，会显著改变土壤环境。丛枝菌根真菌作为植被与土壤之间的媒介和桥梁，在介导植物养分吸收以及调节植被 - 土壤系统的生产力、稳定性和演替过程中发挥着关键作用。为了研究森林火灾对寒温带森林中丛枝菌根真菌群落结构和多样性的影响，我们在中国东北大兴安岭地区，从轻度、中度和重度火灾干扰的森林以及作为对照的天然林中采集了土壤样本。利用Illumina MiSeq技术对丛枝菌根真菌的群落结构和多样性进行测序，并分析其与土壤理化性质的相关性。结果表明，随着火灾强度增加（从轻度火灾到重度火灾），微生物生物量碳（MBC）、含水量（MC）、全氮（TN）和有效磷（AP）含量显著增加（<0.05），但有效钾（AK）显著降低（<0.05）。这些变化并不显著。在所有序列中，共有14554条有效序列被归类为66个可操作分类单元（ASV），它们隶属于1个门、1个目、4个科和4个属。这些属包括、、和，并且在对照林和重度火灾林中是优势属（相对丰度最高的五个属）。非度量多维尺度分析（NMDS）表明，森林火灾显著影响了丛枝菌根真菌的群落结构（<0.01）。冗余分析（RDA）表明，MBC、有机碳（SOC）和AP含量显著影响了丛枝菌根真菌群落的组成结构和多样性。本研究表明，森林火灾通过改变寒温带森林中的土壤理化参数（MBC、SOC和AP），影响了土壤丛枝菌根真菌群落的组成和多样性。对大兴安岭寒温带森林火灾后土壤理化性质和丛枝菌根真菌多样性的研究，为火灾迹地的植被恢复和重建提供了参考依据。
