Wan Qiong-Qiong, Gao Xing-Hui, He Xin-Yuan, Chen Su-Ming, Song Yan-Chao, Gong Qiu-Yu, Li Xiao-Hua, Ma Hui-Min
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (China).
Chem Asian J. 2014 Aug;9(8):2058-62. doi: 10.1002/asia.201402364. Epub 2014 Jun 11.
A new cresyl violet-based fluorescent off-on probe has been developed through a one-step synthesis for the detection of nitroreductase (NTR) and hypoxia. The detection mechanism is based on the NTR-catalyzed reduction of the probe to cresyl violet, accompanied with a large fluorescence enhancement at a long wavelength of 625 nm. The probe can detect NTR in aqueous solution with high selectivity and sensitivity, and the detection limit is 1 ng mL(-1) NTR. Most importantly, the probe has been successfully used to image not only NTR and hypoxia in living cells, but also the distribution of NTR in zebrafish in vivo.
通过一步合成法开发了一种基于新甲酚紫的荧光开-关探针，用于检测硝基还原酶（NTR）和缺氧情况。检测机制基于NTR催化探针还原为甲酚紫，同时在625 nm的长波长处伴随有大幅荧光增强。该探针能够在水溶液中高选择性和高灵敏度地检测NTR，检测限为1 ng mL(-1) NTR。最重要的是，该探针不仅已成功用于对活细胞中的NTR和缺氧情况进行成像，还用于对斑马鱼体内NTR的分布进行成像。
