Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, PR China.
Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 5;188:197-201. doi: 10.1016/j.saa.2017.06.065. Epub 2017 Jul 3.
The biological activity of nitroreductase (NTR) is closely related to biological hypoxia status in organisms. The development of effective methods for monitoring the activity of NTR is of great significance for medical diagnosis and tumor research. Toward this goal, we have developed a new aggregation-induced emission (AIE) fluorescence NTR probe TPE-HY used the tetraphenylethene as the fluorophore, and used the nitro group as the NTR recognition site. The probe TPE-HY has many excellent properties, including rapid response, AIE characteristics, high sensitivity and selectivity, and low cytotoxicity. Importantly, the probe TPE-HY is successfully applied to monitor endogenous NTR in living HeLa cells.
硝基还原酶（NTR）的生物学活性与生物体中的生物缺氧状态密切相关。开发有效监测 NTR 活性的方法对于医学诊断和肿瘤研究具有重要意义。为此，我们以四苯乙烯为荧光团，以硝基为 NTR 识别位点，设计并合成了一种新型聚集诱导发射（AIE）荧光 NTR 探针 TPE-HY。探针 TPE-HY 具有快速响应、AIE 特性、高灵敏度和选择性、低细胞毒性等优异性能。重要的是，该探针 TPE-HY 成功应用于监测活 HeLa 细胞内的内源性 NTR。
