Ow Maria C, Lau Nelson C, Hall Sarah E
Department of Biology, Syracuse University, 110 Life Sciences Complex, 107 College Place, Syracuse, NY, 13244, USA.
Methods Mol Biol. 2014;1173:59-70. doi: 10.1007/978-1-4939-0931-5_6.
In recent years, distinct classes of small RNAs ranging in size from ~21 to 26 nucleotides have been discovered and shown to play important roles in a wide array of cellular functions. Because of the abundance of these small RNAs, library preparation from an RNA sample followed by deep sequencing provides the identity and quantity of a particular class of small RNAs. In this chapter we describe a detailed protocol for preparing small RNA libraries for deep sequencing on the Illumina platform from the nematode C. elegans.
近年来，人们发现了大小在约21至26个核苷酸之间的不同类别的小RNA，并表明它们在广泛的细胞功能中发挥着重要作用。由于这些小RNA数量丰富，从RNA样本中制备文库并进行深度测序可提供特定类别的小RNA的身份和数量。在本章中，我们描述了一种详细的方案，用于从线虫秀丽隐杆线虫中制备小RNA文库，以便在Illumina平台上进行深度测序。
