Vosough Maryam, Mohamedian Hadi, Salemi Amir, Baheri Tahmineh
Chemistry and Chemical Engineering Research Centre of Iran, Tehran, Iran
Chemistry and Chemical Engineering Research Centre of Iran, Tehran, Iran.
J Chromatogr Sci. 2015 Feb;53(2):233-9. doi: 10.1093/chromsci/bmu046. Epub 2014 Jun 11.
In the present study, a simple strategy based on solid-phase extraction (SPE) with a cation exchange sorbent (Finisterre SCX) followed by fast high-performance liquid chromatography (HPLC) with diode array detection coupled with chemometrics tools has been proposed for the determination of methamphetamine and pseudoephedrine in ground water and river water. At first, the HPLC and SPE conditions were optimized and the analytical performance of the method was determined. In the case of ground water, determination of analytes was successfully performed through univariate calibration curves. For river water sample, multivariate curve resolution and alternating least squares was implemented and the second-order advantage was achieved in samples containing uncalibrated interferences and uncorrected background signals. The calibration curves showed good linearity (r(2) > 0.994).The limits of detection for pseudoephedrine and methamphetamine were 0.06 and 0.08 μg/L and the average recovery values were 104.7 and 102.3% in river water, respectively.
在本研究中，提出了一种基于阳离子交换吸附剂（菲尼斯特雷SCX）固相萃取（SPE），随后进行二极管阵列检测的快速高效液相色谱（HPLC）并结合化学计量学工具的简单策略，用于测定地下水和河水中的甲基苯丙胺和伪麻黄碱。首先，对HPLC和SPE条件进行了优化，并确定了该方法的分析性能。对于地下水，通过单变量校准曲线成功地进行了分析物的测定。对于河水样品，实施了多元曲线分辨和交替最小二乘法，在含有未校准干扰和未校正背景信号的样品中实现了二阶优势。校准曲线显示出良好的线性（r(2) > 0.994）。伪麻黄碱和甲基苯丙胺的检测限分别为0.06和0.08 μg/L，在河水中的平均回收率分别为104.7%和102.3%。
